Detailed information of TA system TA060014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1203456..1203994
• Replicon: chromosome
• Accession: NZ_CP014042
• Organism: Vibrio mimicus strain FDAARGOS_112

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AL543_RS06065
• Protein ID: WP_061051377.1
• Coordinates: 1203695..1203994 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A4Q5K3S2
• Locus tag: AL543_RS06060
• Protein ID: WP_017019247.1
• Coordinates: 1203456..1203698 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL543_RS06040	1200483..1201442	+	960	WP_042988413.1	IS30 family transposase	-
AL543_RS06045	1201473..1201733	-	261	WP_061051486.1	hypothetical protein	-
AL543_RS21525	1202021..1202098	-	78	WP_123772164.1	hypothetical protein	-
AL543_RS06050	1202136..1202438	-	303	WP_038969944.1	hypothetical protein	-
AL543_RS06055	1202586..1203188	-	603	WP_123772162.1	hypothetical protein	-
AL543_RS06060	1203456..1203698	+	243	WP_017019247.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
AL543_RS06065	1203695..1203994	+	300	WP_061051377.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL543_RS06070	1204124..1204411	-	288	WP_061051378.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AL543_RS06075	1204408..1204689	-	282	WP_000086650.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AL543_RS06080	1205216..1205416	-	201	WP_104408352.1	DUF3265 domain-containing protein	-
AL543_RS06085	1205434..1205793	-	360	WP_061051379.1	VOC family protein	-
AL543_RS06090	1206078..1206311	-	234	WP_081096082.1	DUF3709 domain-containing protein	-
AL543_RS21530	1206332..1206537	-	206	Protein_1172	DUF3709 domain-containing protein	-
AL543_RS21535	1206585..1206785	-	201	WP_025630366.1	hypothetical protein	-
AL543_RS06100	1207244..1207591	-	348	WP_017036770.1	DUF3024 domain-containing protein	-
AL543_RS06105	1207648..1207794	-	147	WP_081096124.1	DUF3265 domain-containing protein	-
AL543_RS06110	1207799..1208383	-	585	WP_188318526.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1086679..1234651	147972
	-	inside	Integron	-	-	1108123..1230683	122560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11492.21 Da
• Isoelectric Point: 9.8924

>T60014 WP_061051377.1 NZ_CP014042:1203695-1203994 [Vibrio mimicus]
MKPFTLTISAKNDLKDVAIFTARRWGKEQRNIYLKQFDDSFWLLAENPDIGKGCDEIRNGYRKFPQGSHVIFYQQTGSQQ
IQIIRILHKSMDISPIFGA

Nucleotide

• Length: 300 bp

>T60014 NZ_CP014042:1203695-1203994 [Vibrio mimicus]
ATGAAGCCATTCACATTAACGATTTCAGCAAAAAATGATCTTAAGGACGTAGCTATATTTACGGCTCGTCGTTGGGGAAA
AGAGCAACGAAATATTTACCTTAAACAGTTTGATGATTCTTTTTGGTTGTTAGCGGAAAATCCAGATATAGGTAAAGGCT
GTGATGAAATTAGGAATGGTTATCGAAAATTCCCCCAAGGTAGCCACGTAATATTTTACCAACAAACAGGTAGCCAACAA
ATACAGATAATTAGAATCCTGCACAAAAGTATGGACATCTCCCCTATTTTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8881.71 Da
• Isoelectric Point: 4.3077

>AT60014 WP_017019247.1 NZ_CP014042:1203456-1203698 [Vibrio mimicus]
MAKNTSITLGDHFEGFISSQIQSGRYGSTSEVIRSALRLLETQETKLHTLRNSLIEGEESGVADYDLDSFINELDSENSK

Nucleotide

• Length: 243 bp

>AT60014 NZ_CP014042:1203456-1203698 [Vibrio mimicus]
ATGGCTAAAAACACAAGTATTACTTTAGGTGATCATTTTGAAGGCTTTATTAGCTCACAAATTCAATCTGGAAGATACGG
CTCAACCAGTGAGGTTATTCGATCTGCGTTACGTTTACTTGAAACACAAGAAACAAAATTGCATACATTACGTAACTCTC
TTATTGAAGGAGAAGAAAGCGGGGTCGCTGATTACGATCTAGATTCATTTATTAATGAACTTGATAGCGAAAATTCAAAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A4Q5K3S2