Detailed information of TA system TA059994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4444056..4444687
• Replicon: chromosome
• Accession: NZ_CP014031
• Organism: Hafnia paralvei strain FDAARGOS_158

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AL518_RS20420
• Protein ID: WP_061059845.1
• Coordinates: 4444514..4444687 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AL518_RS20415
• Protein ID: WP_061060361.1
• Coordinates: 4444056..4444463 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL518_RS20380	4440322..4440816	-	495	WP_004092442.1	DUF1441 family protein	-
AL518_RS20385	4441031..4441234	-	204	WP_061060356.1	hypothetical protein	-
AL518_RS20390	4441346..4441870	-	525	WP_061060357.1	DUF2514 domain-containing protein	-
AL518_RS20395	4441867..4442397	-	531	WP_061060358.1	lysozyme	-
AL518_RS20400	4442397..4442666	-	270	Protein_3879	lysis protein	-
AL518_RS20405	4442852..4443322	+	471	WP_061060359.1	2TM domain-containing protein	-
AL518_RS20410	4443496..4443915	-	420	WP_061060360.1	hypothetical protein	-
AL518_RS20415	4444056..4444463	-	408	WP_061060361.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AL518_RS20420	4444514..4444687	-	174	WP_061059845.1	type II toxin-antitoxin system HicA family toxin	Toxin
AL518_RS20425	4444825..4445088	+	264	WP_061060362.1	hypothetical protein	-
AL518_RS20430	4445199..4445894	-	696	WP_061060363.1	hypothetical protein	-
AL518_RS20435	4445915..4446913	-	999	WP_061060364.1	DUF968 domain-containing protein	-
AL518_RS20440	4446910..4447557	-	648	WP_061060365.1	phage N-6-adenine-methyltransferase	-
AL518_RS20445	4447560..4447832	-	273	WP_061060366.1	PerC family transcriptional regulator	-
AL518_RS20450	4447829..4448785	-	957	WP_061060367.1	helix-turn-helix domain-containing protein	-
AL518_RS20455	4448782..4448961	-	180	WP_061059837.1	DUF4222 domain-containing protein	-
AL518_RS20460	4449142..4449675	-	534	WP_061060368.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4421928..4457229	35301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-63)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6561.67 Da
• Isoelectric Point: 11.6500

>T59994 WP_061059845.1 NZ_CP014031:c4444687-4444514 [Hafnia paralvei]
VKQSEFRRWLEAQGVEVSNGTNHLKLRLNGKRSVMPRHPSSEIKEPLRKAIIKQLGL

Nucleotide

• Length: 174 bp

>T59994 NZ_CP014031:c4444687-4444514 [Hafnia paralvei]
GTGAAGCAAAGCGAGTTTCGAAGATGGCTCGAAGCTCAGGGGGTTGAGGTTTCAAATGGTACGAATCACTTGAAGCTAAG
ACTAAACGGGAAGCGAAGTGTAATGCCAAGGCATCCCAGCTCTGAGATAAAAGAGCCGCTAAGAAAAGCAATCATCAAGC
AGTTAGGATTGTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14978.25 Da
• Isoelectric Point: 4.4949

>AT59994 WP_061060361.1 NZ_CP014031:c4444463-4444056 [Hafnia paralvei]
MRYPVNLEPDSGGFFVSFPDIPEALTQGDGREDALSMALDALVTAFEFYFEDNRKVPLPSEVTGEFVDVPLSVASKVLML
NAFIDSGLTQTELANRMGVKKQEITRLFDLKHATKIDAIQKAMQALGKRLELQVI

Nucleotide

• Length: 408 bp

>AT59994 NZ_CP014031:c4444463-4444056 [Hafnia paralvei]
ATGCGATATCCCGTAAATCTAGAACCAGACAGCGGTGGATTTTTCGTAAGCTTCCCAGATATACCGGAAGCACTAACACA
GGGCGATGGCCGCGAAGACGCGTTATCAATGGCGCTCGATGCTTTAGTTACGGCTTTTGAGTTCTATTTCGAAGATAACC
GGAAAGTACCGCTACCCAGTGAGGTAACAGGGGAGTTTGTTGATGTGCCGCTTAGTGTGGCCTCAAAAGTGCTTATGCTT
AATGCTTTTATTGATTCCGGCTTGACGCAAACCGAACTGGCTAATCGAATGGGGGTCAAGAAACAAGAGATCACCCGGCT
ATTTGATCTAAAGCATGCGACTAAAATCGATGCAATTCAGAAAGCTATGCAGGCTCTCGGTAAGAGGTTAGAGCTCCAAG
TCATTTAG