Detailed information of TA system TA059965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 449057..449673
• Replicon: chromosome
• Accession: NZ_CP014030
• Organism: Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AL515_RS02200
• Protein ID: WP_061066953.1
• Coordinates: 449299..449673 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B5NTK4
• Locus tag: AL515_RS02195
• Protein ID: WP_043018956.1
• Coordinates: 449057..449299 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL515_RS02160	444186..444785	+	600	WP_048214033.1	glucose-1-phosphatase	-
AL515_RS02165	444779..445651	+	873	WP_048214032.1	virulence factor BrkB family protein	-
AL515_RS02170	445648..446085	+	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AL515_RS02175	446130..447071	+	942	WP_048214031.1	fatty acid biosynthesis protein FabY	-
AL515_RS02180	447083..447529	-	447	WP_048214030.1	helix-turn-helix domain-containing protein	-
AL515_RS02185	447526..447837	-	312	WP_061066951.1	type II toxin-antitoxin system HigB family toxin	-
AL515_RS02190	447923..448852	-	930	WP_061066952.1	alpha/beta hydrolase	-
AL515_RS02195	449057..449299	+	243	WP_043018956.1	ribbon-helix-helix domain-containing protein	Antitoxin
AL515_RS02200	449299..449673	+	375	WP_061066953.1	type II toxin-antitoxin system VapC family toxin	Toxin
AL515_RS02205	449710..450639	-	930	WP_061066954.1	formate dehydrogenase accessory protein FdhE	-
AL515_RS02210	450636..451271	-	636	WP_061066955.1	formate dehydrogenase cytochrome b556 subunit	-
AL515_RS02215	451268..452170	-	903	WP_003028691.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13672.88 Da
• Isoelectric Point: 9.5622

>T59965 WP_061066953.1 NZ_CP014030:449299-449673 [Citrobacter sp. FDAARGOS_156]
MVKGSALFDTNILIDLFSGRSEAKQAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLPGVVTPYTL

Nucleotide

• Length: 375 bp

>T59965 NZ_CP014030:449299-449673 [Citrobacter sp. FDAARGOS_156]
ATGGTCAAAGGATCCGCACTTTTTGACACCAACATCCTCATCGACCTGTTTAGTGGACGGAGTGAAGCAAAGCAAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTACCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9451.75 Da
• Isoelectric Point: 4.3437

>AT59965 WP_043018956.1 NZ_CP014030:449057-449299 [Citrobacter sp. FDAARGOS_156]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT59965 NZ_CP014030:449057-449299 [Citrobacter sp. FDAARGOS_156]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTGCGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGCGTTGAGTACCAACGCAAACTTCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6B5NTK4