Detailed information of TA system TA059944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 213974..214690
• Replicon: chromosome
• Accession: NZ_CP014025
• Organism: Pseudomonas fulva strain FDAARGOS_167

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AL527_RS00880
• Protein ID: WP_080992932.1
• Coordinates: 214508..214690 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0S4I868
• Locus tag: AL527_RS00875
• Protein ID: WP_049696767.1
• Coordinates: 213974..214420 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL527_RS00860	211227..211376	-	150	Protein_158	DUF3079 domain-containing protein	-
AL527_RS00865	211714..212526	+	813	WP_049696769.1	alpha/beta hydrolase	-
AL527_RS00870	212519..213676	+	1158	WP_104925706.1	HAMP domain-containing histidine kinase	-
AL527_RS00875	213974..214420	-	447	WP_049696767.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AL527_RS00880	214508..214690	-	183	WP_080992932.1	type II toxin-antitoxin system HicA family toxin	Toxin
AL527_RS00890	216068..218116	-	2049	WP_104925707.1	RNA-directed DNA polymerase	-
AL527_RS00895	218109..219455	-	1347	WP_104925708.1	RNA-directed DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6809.81 Da
• Isoelectric Point: 11.4650

>T59944 WP_080992932.1 NZ_CP014025:c214690-214508 [Pseudomonas fulva]
VHSRDIIKRLKDDGWVRVKGTDGSHQQFKHPTKKGRVTVPHPNKDVATGTLRNIWKQAGL

Nucleotide

• Length: 183 bp

>T59944 NZ_CP014025:c214690-214508 [Pseudomonas fulva]
GTGCACAGCCGAGACATCATCAAGCGGCTGAAGGATGACGGTTGGGTCAGAGTCAAAGGGACTGATGGGAGCCACCAGCA
GTTCAAGCATCCAACCAAGAAGGGGCGGGTTACGGTTCCTCATCCAAATAAGGATGTGGCAACCGGGACGCTTCGAAACA
TTTGGAAGCAAGCCGGTCTCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16381.38 Da
• Isoelectric Point: 4.3924

>AT59944 WP_049696767.1 NZ_CP014025:c214420-213974 [Pseudomonas fulva]
MKYPVVLHKDEDSDYGVTVPDVPGCFSAGETIDEALSEVQLALEMHFEGLVEDGEPLPQPQNIEIHRKDPAYADGIWAFV
EFDVTPYYGKSVRFNASLPEGLLKRIDDRVKEDSAYASRSGFLAAAALHELAKNLNEGIDALKTRKTV

Nucleotide

• Length: 447 bp

>AT59944 NZ_CP014025:c214420-213974 [Pseudomonas fulva]
ATGAAATACCCAGTTGTTTTGCACAAAGATGAAGACTCCGACTACGGAGTGACAGTTCCAGACGTACCGGGCTGCTTCTC
TGCTGGCGAAACGATCGATGAAGCATTGAGCGAAGTCCAACTCGCGTTAGAGATGCACTTTGAAGGTTTGGTAGAAGATG
GAGAGCCTCTGCCGCAACCTCAAAATATCGAAATCCATAGGAAGGACCCGGCTTATGCCGATGGCATCTGGGCTTTCGTA
GAATTCGACGTCACCCCCTACTATGGGAAGTCGGTTCGCTTTAACGCGTCTTTACCAGAAGGTCTCCTCAAACGCATAGA
TGATCGCGTGAAAGAAGACAGCGCTTATGCGTCCAGATCAGGATTTTTAGCCGCGGCGGCGCTGCATGAGCTGGCGAAAA
ACTTAAACGAAGGCATAGACGCTTTAAAAACCAGAAAGACAGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4I868