Detailed information of TA system TA059909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 394564..395078
• Replicon: chromosome
• Accession: NZ_CP014016
• Organism: Staphylococcus simulans strain FDAARGOS_124

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AL483_RS14720
• Protein ID: WP_031262808.1
• Coordinates: 394728..395078 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4QNU3
• Locus tag: AL483_RS14715
• Protein ID: WP_023016097.1
• Coordinates: 394564..394731 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL483_RS14690	390455..390919	+	465	WP_060804059.1	PH domain-containing protein	-
AL483_RS14695	390912..392404	+	1493	Protein_390	PH domain-containing protein	-
AL483_RS14700	392406..392894	+	489	WP_105165371.1	PH domain-containing protein	-
AL483_RS14705	392896..393249	+	354	WP_031262810.1	holo-ACP synthase	-
AL483_RS14710	393329..394483	+	1155	WP_002481752.1	alanine racemase	-
AL483_RS14715	394564..394731	+	168	WP_023016097.1	hypothetical protein	Antitoxin
AL483_RS14720	394728..395078	+	351	WP_031262808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL483_RS14725	395370..396371	+	1002	WP_002481750.1	PP2C family protein-serine/threonine phosphatase	-
AL483_RS14730	396447..396773	+	327	WP_002481749.1	anti-sigma factor antagonist	-
AL483_RS14735	396775..397254	+	480	WP_023016096.1	anti-sigma B factor RsbW	-
AL483_RS14740	397229..397999	+	771	WP_002481747.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.09 Da
• Isoelectric Point: 10.0474

>T59909 WP_031262808.1 NZ_CP014016:394728-395078 [Staphylococcus simulans]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIGISLGLTHKN

Nucleotide

• Length: 351 bp

>T59909 NZ_CP014016:394728-395078 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAGGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6257.78 Da
• Isoelectric Point: 3.6836

>AT59909 WP_023016097.1 NZ_CP014016:394564-394731 [Staphylococcus simulans]
MSTFNQNRSYSLEQSLKEGYSQMADLNLSLATEAFSVECEACDCNESYLTFDKDE

Nucleotide

• Length: 168 bp

>AT59909 NZ_CP014016:394564-394731 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4QNU3