Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3168180..3168800 Replicon chromosome
Accession NZ_CP014015
Organism Citrobacter amalonaticus strain FDAARGOS_122

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag AL479_RS15270 Protein ID WP_001280991.1
Coordinates 3168180..3168398 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag AL479_RS15275 Protein ID WP_042324455.1
Coordinates 3168426..3168800 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL479_RS15240 3163831..3164427 + 597 WP_061076655.1 membrane protein -
AL479_RS15245 3164510..3164863 + 354 WP_061076656.1 DUF1428 family protein -
AL479_RS15250 3164903..3166453 - 1551 WP_061076657.1 EAL domain-containing protein -
AL479_RS15255 3166676..3166816 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AL479_RS15260 3166868..3167338 - 471 WP_061076658.1 YlaC family protein -
AL479_RS15265 3167452..3168003 - 552 WP_061076659.1 maltose O-acetyltransferase -
AL479_RS15270 3168180..3168398 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
AL479_RS15275 3168426..3168800 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
AL479_RS15280 3169313..3172462 - 3150 WP_061076660.1 efflux RND transporter permease AcrB -
AL479_RS15285 3172485..3173678 - 1194 WP_061076661.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T59897 WP_001280991.1 NZ_CP014015:c3168398-3168180 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T59897 NZ_CP014015:c3168398-3168180 [Citrobacter amalonaticus]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTGCGACGCTGTCAGACAATTGACACGCTTGAGCGCGT
GATCGAGAAAAATAAATATGAATTATCTGACAATGAACTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTCGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT59897 WP_042324455.1 NZ_CP014015:c3168800-3168426 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT59897 NZ_CP014015:c3168800-3168426 [Citrobacter amalonaticus]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGCTGCTTTGTGAATGCCACCAGAGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References