Detailed information of TA system TA059896

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 2991943..2992688
• Replicon: chromosome
• Accession: NZ_CP014015
• Organism: Citrobacter amalonaticus strain FDAARGOS_122

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AL479_RS14460
• Protein ID: WP_061076549.1
• Coordinates: 2992320..2992688 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6L5E7I7
• Locus tag: AL479_RS14455
• Protein ID: WP_057101183.1
• Coordinates: 2991943..2992299 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL479_RS14420	2987982..2988698	+	717	WP_049082522.1	WYL domain-containing protein	-
AL479_RS14425	2988734..2989186	+	453	WP_049082528.1	hypothetical protein	-
AL479_RS14430	2989258..2989731	+	474	WP_061076545.1	hypothetical protein	-
AL479_RS14435	2989852..2990673	+	822	WP_061076546.1	DUF945 domain-containing protein	-
AL479_RS14440	2990704..2991144	+	441	WP_061076547.1	antirestriction protein	-
AL479_RS14445	2991157..2991699	+	543	WP_061076548.1	DNA repair protein RadC	-
AL479_RS14450	2991696..2991917	+	222	WP_057101182.1	DUF987 domain-containing protein	-
AL479_RS14455	2991943..2992299	+	357	WP_057101183.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AL479_RS14460	2992320..2992688	+	369	WP_061076549.1	TA system toxin CbtA family protein	Toxin
AL479_RS14470	2993316..2994212	+	897	WP_061076550.1	helix-turn-helix transcriptional regulator	-
AL479_RS14475	2994471..2995775	+	1305	WP_061076551.1	glycoside-pentoside-hexuronide (GPH):cation symporter	-
AL479_RS14480	2995837..2997432	+	1596	WP_061076552.1	glycoside hydrolase family 43 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2952233..2994212	41979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13827.10 Da
• Isoelectric Point: 8.7200

>T59896 WP_061076549.1 NZ_CP014015:2992320-2992688 [Citrobacter amalonaticus]
MKFLPATNMRAAKPCLTPVTIWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVHAPYLTATDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T59896 NZ_CP014015:2992320-2992688 [Citrobacter amalonaticus]
ATGAAATTTCTACCTGCAACAAATATGCGGGCGGCGAAGCCATGCCTGACGCCCGTGACTATCTGGCAAATGTTACTTAC
TCGTCTGCTGGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCCGGTATCACCCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACATGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13002.74 Da
• Isoelectric Point: 6.0590

>AT59896 WP_057101183.1 NZ_CP014015:2991943-2992299 [Citrobacter amalonaticus]
MSIHSDSGTKPENPASQPWGLKRAITPCFGARLVQEGSRVHFLADRAGFNGAFSDEDALSLDQAFPLILKQLELMLISGE
LNPRYQHSVMLYHNGLTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 357 bp

>AT59896 NZ_CP014015:2991943-2992299 [Citrobacter amalonaticus]
ATGAGCATTCACTCAGACTCCGGTACCAAGCCGGAGAATCCCGCCAGCCAGCCGTGGGGACTTAAACGCGCTATTACACC
CTGTTTTGGAGCTCGTCTGGTACAGGAAGGCAGCCGGGTGCATTTTCTCGCTGACCGCGCCGGGTTTAATGGTGCTTTCA
GCGACGAGGATGCGTTGAGCCTCGACCAGGCATTTCCATTAATACTCAAACAACTGGAACTAATGCTCATCAGTGGCGAG
CTCAATCCCCGCTATCAACACAGCGTCATGCTTTACCACAACGGACTCACCTGCGAAGCCGATACGCTTGGTAGTTGTGG
CTACGTATACATCGCCGTTTATCCCACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6L5E7I7