Detailed information of TA system TA059888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2599954..2600485
• Replicon: chromosome
• Accession: NZ_CP014013
• Organism: Bordetella bronchiseptica strain FDAARGOS_176

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: AL472_RS12375
• Protein ID: WP_003812626.1
• Coordinates: 2600219..2600485 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: AL472_RS12370
• Protein ID: WP_003816743.1
• Coordinates: 2599954..2600232 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL472_RS12345	2595377..2596471	+	1095	WP_010926390.1	TRAP transporter substrate-binding protein	-
AL472_RS12350	2596683..2597312	+	630	WP_003812635.1	TRAP transporter small permease subunit	-
AL472_RS12355	2597315..2598982	+	1668	WP_003812634.1	TRAP transporter large permease subunit	-
AL472_RS12360	2598997..2599653	+	657	WP_010926391.1	phosphoribosylanthranilate isomerase	-
AL472_RS12370	2599954..2600232	-	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
AL472_RS12375	2600219..2600485	-	267	WP_003812626.1	BrnT family toxin	Toxin
AL472_RS12380	2600716..2602257	-	1542	WP_010926392.1	M81 family metallopeptidase	-
AL472_RS12385	2602319..2603197	-	879	WP_010926393.1	tripartite tricarboxylate transporter substrate binding protein	-
AL472_RS12390	2603594..2604226	+	633	WP_003812619.1	PAS domain-containing protein	-
AL472_RS12395	2604337..2605047	+	711	WP_003812617.1	aquaporin Z	-
AL472_RS12400	2605086..2605358	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T59888 WP_003812626.1 NZ_CP014013:c2600485-2600219 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T59888 NZ_CP014013:c2600485-2600219 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT59888 WP_003816743.1 NZ_CP014013:c2600232-2599954 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT59888 NZ_CP014013:c2600232-2599954 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02