Detailed information of TA system TA059885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 52711..53381
• Replicon: plasmid pRJF999
• Accession: NZ_CP014011
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF999

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: RJF9_RS26965
• Protein ID: WP_004213072.1
• Coordinates: 52711..53154 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: RJF9_RS26970
• Protein ID: WP_004213073.1
• Coordinates: 53151..53381 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF9_RS26935	48121..48396	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
RJF9_RS26940	48459..48950	+	492	WP_011251327.1	DM13 domain-containing protein	-
RJF9_RS26945	48999..49919	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
RJF9_RS29215	50010..50416	+	407	Protein_55	GAF domain-containing protein	-
RJF9_RS31280	50931..51567	-	637	Protein_56	mucoid phenotype A regulator RmpA	-
RJF9_RS26955	51984..52277	+	294	Protein_57	transposase	-
RJF9_RS26960	52281..52697	+	417	Protein_58	restriction endonuclease	-
RJF9_RS26965	52711..53154	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJF9_RS26970	53151..53381	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RJF9_RS26975	53989..55122	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
RJF9_RS26980	55138..55431	+	294	WP_004213076.1	hypothetical protein	-
RJF9_RS26985	55421..55627	-	207	WP_004213077.1	hypothetical protein	-
RJF9_RS26990	55979..56269	+	291	WP_004213078.1	hypothetical protein	-
RJF9_RS26995	56259..57158	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228907	228907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T59885 WP_004213072.1 NZ_CP014011:c53154-52711 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T59885 NZ_CP014011:c53154-52711 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT59885 WP_004213073.1 NZ_CP014011:c53381-53151 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT59885 NZ_CP014011:c53381-53151 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620