Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1272588..1273207 Replicon chromosome
Accession NZ_CP014010
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJF999

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag RJF9_RS06260 Protein ID WP_002892050.1
Coordinates 1272588..1272806 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag RJF9_RS06265 Protein ID WP_002892066.1
Coordinates 1272833..1273207 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJF9_RS06225 1268635..1268898 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
RJF9_RS06230 1268898..1269038 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
RJF9_RS06235 1269035..1269733 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
RJF9_RS06240 1269834..1271285 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
RJF9_RS06245 1271260..1271730 - 471 WP_002892026.1 YlaC family protein -
RJF9_RS30480 1271751..1271891 + 141 WP_004147370.1 hypothetical protein -
RJF9_RS06255 1271863..1272429 - 567 WP_002892030.1 maltose O-acetyltransferase -
RJF9_RS06260 1272588..1272806 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
RJF9_RS06265 1272833..1273207 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
RJF9_RS06270 1273693..1276839 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
RJF9_RS06275 1276862..1278055 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T59871 WP_002892050.1 NZ_CP014010:c1272806-1272588 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T59871 NZ_CP014010:c1272806-1272588 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT59871 WP_002892066.1 NZ_CP014010:c1273207-1272833 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT59871 NZ_CP014010:c1273207-1272833 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References