Detailed information of TA system TA059867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 211730..212400
• Replicon: plasmid pRJF293
• Accession: NZ_CP014009
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: RJF2_RS26620
• Protein ID: WP_004213072.1
• Coordinates: 211730..212173 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: RJF2_RS26625
• Protein ID: WP_004213073.1
• Coordinates: 212170..212400 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF2_RS26590	207139..207414	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
RJF2_RS26595	207477..207968	+	492	WP_011251327.1	DM13 domain-containing protein	-
RJF2_RS26600	208017..208937	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
RJF2_RS28035	209028..209434	+	407	Protein_221	GAF domain-containing protein	-
RJF2_RS29455	209950..210586	-	637	Protein_222	response regulator	-
RJF2_RS26610	211003..211296	+	294	Protein_223	transposase	-
RJF2_RS26615	211300..211716	+	417	Protein_224	restriction endonuclease	-
RJF2_RS26620	211730..212173	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJF2_RS26625	212170..212400	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RJF2_RS26630	213008..214141	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
RJF2_RS26635	214157..214450	+	294	WP_004213076.1	hypothetical protein	-
RJF2_RS26640	214440..214646	-	207	WP_004213077.1	hypothetical protein	-
RJF2_RS26645	214998..215288	+	291	WP_004213078.1	hypothetical protein	-
RJF2_RS26650	215278..216177	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..224263	224263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T59867 WP_004213072.1 NZ_CP014009:c212173-211730 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T59867 NZ_CP014009:c212173-211730 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT59867 WP_004213073.1 NZ_CP014009:c212400-212170 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT59867 NZ_CP014009:c212400-212170 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620