Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4410664..4411321 Replicon chromosome
Accession NZ_CP014008
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag RJF2_RS21425 Protein ID WP_002916310.1
Coordinates 4410664..4411074 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag RJF2_RS21430 Protein ID WP_002916312.1
Coordinates 4411055..4411321 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJF2_RS21405 4406664..4408397 - 1734 WP_060176113.1 single-stranded-DNA-specific exonuclease RecJ -
RJF2_RS21410 4408403..4409116 - 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RJF2_RS21415 4409139..4410035 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
RJF2_RS21420 4410136..4410657 + 522 WP_004144730.1 flavodoxin FldB -
RJF2_RS21425 4410664..4411074 - 411 WP_002916310.1 protein YgfX Toxin
RJF2_RS21430 4411055..4411321 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
RJF2_RS21435 4411567..4412550 + 984 WP_023286864.1 tRNA-modifying protein YgfZ -
RJF2_RS21440 4412701..4413360 - 660 WP_002916317.1 hemolysin III family protein -
RJF2_RS21445 4413524..4413835 - 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
RJF2_RS21450 4413885..4414613 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
RJF2_RS21455 4414732..4416165 + 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T59861 WP_002916310.1 NZ_CP014008:c4411074-4410664 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T59861 NZ_CP014008:c4411074-4410664 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATTCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT59861 WP_002916312.1 NZ_CP014008:c4411321-4411055 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT59861 NZ_CP014008:c4411321-4411055 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References