59851

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1244182..1244801 Replicon chromosome
Accession NZ_CP014008
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag RJF2_RS06110 Protein ID WP_002892050.1
Coordinates 1244182..1244400 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag RJF2_RS06115 Protein ID WP_002892066.1
Coordinates 1244427..1244801 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJF2_RS06075 1240229..1240492 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
RJF2_RS06080 1240492..1240632 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
RJF2_RS06085 1240629..1241327 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
RJF2_RS06090 1241428..1242879 + 1452 WP_060175626.1 PLP-dependent aminotransferase family protein -
RJF2_RS06095 1242854..1243324 - 471 WP_002892026.1 YlaC family protein -
RJF2_RS29025 1243345..1243485 + 141 WP_004147370.1 hypothetical protein -
RJF2_RS06105 1243457..1244023 - 567 WP_002892030.1 maltose O-acetyltransferase -
RJF2_RS06110 1244182..1244400 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
RJF2_RS06115 1244427..1244801 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
RJF2_RS06120 1245287..1248433 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
RJF2_RS06125 1248456..1249649 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T59851 WP_002892050.1 NZ_CP014008:c1244400-1244182 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T59851 NZ_CP014008:c1244400-1244182 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT59851 WP_002892066.1 NZ_CP014008:c1244801-1244427 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT59851 NZ_CP014008:c1244801-1244427 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References