Detailed information of TA system TA059849

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 600331..601034
• Replicon: chromosome
• Accession: NZ_CP014008
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: RJF2_RS02960
• Protein ID: WP_060175539.1
• Coordinates: 600693..601034 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: RJF2_RS02955
• Protein ID: WP_060175538.1
• Coordinates: 600331..600672 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF2_RS02920	595486..596361	+	876	WP_060175533.1	GTPase family protein	-
RJF2_RS26855	596605..597321	+	717	WP_012543038.1	WYL domain-containing protein	-
RJF2_RS02930	597357..597809	+	453	WP_060175534.1	hypothetical protein	-
RJF2_RS02935	597880..598353	+	474	WP_060175535.1	hypothetical protein	-
RJF2_RS02940	598473..599294	+	822	WP_060175536.1	DUF945 domain-containing protein	-
RJF2_RS02945	599325..599765	+	441	WP_060175537.1	antirestriction protein	-
RJF2_RS02950	599778..600320	+	543	WP_017880113.1	DNA repair protein RadC	-
RJF2_RS02955	600331..600672	+	342	WP_060175538.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RJF2_RS02960	600693..601034	+	342	WP_060175539.1	TA system toxin CbtA family protein	Toxin
RJF2_RS02965	601299..602306	+	1008	WP_060175540.1	restriction endonuclease	-
RJF2_RS28425	602681..602791	+	111	Protein_537	DUF4102 domain-containing protein	-
RJF2_RS28430	602804..602938	-	135	Protein_538	transposase	-
RJF2_RS02975	603084..603347	-	264	WP_004192273.1	hypothetical protein	-
RJF2_RS02980	603337..604464	-	1128	WP_023325431.1	PAAR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	564594..609498	44904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12760.77 Da
• Isoelectric Point: 9.6548

>T59849 WP_060175539.1 NZ_CP014008:600693-601034 [Klebsiella pneumoniae subsp. pneumoniae]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLKRNRISAVR

Nucleotide

• Length: 342 bp

>T59849 NZ_CP014008:600693-601034 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAATGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGTAGGGGATTC
AGCTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGTACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12726.38 Da
• Isoelectric Point: 6.7345

>AT59849 WP_060175538.1 NZ_CP014008:600331-600672 [Klebsiella pneumoniae subsp. pneumoniae]
MKSTDSENDSFLKWGLNRNLTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT59849 NZ_CP014008:600331-600672 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATTTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTAGATGTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA