Detailed information of TA system TA059847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 512189..512705
• Replicon: chromosome
• Accession: NZ_CP014008
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RJF2_RS02545
• Protein ID: WP_060175501.1
• Coordinates: 512421..512705 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: RJF2_RS02540
• Protein ID: WP_002886901.1
• Coordinates: 512189..512431 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF2_RS02525	508217..508957	+	741	WP_060175498.1	KDGP aldolase family protein	-
RJF2_RS02530	509024..510178	+	1155	WP_060175499.1	lactonase family protein	-
RJF2_RS02535	510201..512111	+	1911	WP_060175500.1	BglG family transcription antiterminator	-
RJF2_RS02540	512189..512431	+	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
RJF2_RS02545	512421..512705	+	285	WP_060175501.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RJF2_RS02550	512709..513173	-	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
RJF2_RS02555	513422..515560	-	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
RJF2_RS02565	515917..516660	-	744	WP_060175502.1	MurR/RpiR family transcriptional regulator	-
RJF2_RS28945	516663..516836	-	174	WP_060175503.1	hypothetical protein	-
RJF2_RS02575	516921..517229	+	309	WP_002886907.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11184.03 Da
• Isoelectric Point: 10.3787

>T59847 WP_060175501.1 NZ_CP014008:512421-512705 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKVSGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T59847 NZ_CP014008:512421-512705 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGTGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT59847 WP_002886901.1 NZ_CP014008:512189-512431 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT59847 NZ_CP014008:512189-512431 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0