Detailed information of TA system TA059822

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3008190..3009016
• Replicon: chromosome
• Accession: NZ_CP014004
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain NUHL24835

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AWN66_RS14700
• Protein ID: WP_000854683.1
• Coordinates: 3008190..3008564 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AWN66_RS14705
• Protein ID: WP_024186744.1
• Coordinates: 3008654..3009016 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN66_RS14665	3003460..3003726	+	267	WP_004178415.1	hypothetical protein	-
AWN66_RS14670	3003726..3004398	+	673	Protein_2841	DUF4400 domain-containing protein	-
AWN66_RS28165	3004409..3005294	+	886	Protein_2842	RES domain-containing protein	-
AWN66_RS29340	3005493..3005681	-	189	Protein_2843	transposase	-
AWN66_RS28985	3005698..3006809	-	1112	Protein_2844	IS3 family transposase	-
AWN66_RS14690	3007493..3007735	-	243	WP_074420605.1	DUF957 domain-containing protein	-
AWN66_RS14695	3007702..3008193	-	492	WP_000976831.1	hypothetical protein	-
AWN66_RS14700	3008190..3008564	-	375	WP_000854683.1	TA system toxin CbtA family protein	Toxin
AWN66_RS14705	3008654..3009016	-	363	WP_024186744.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AWN66_RS14710	3009095..3009316	-	222	WP_000692312.1	DUF987 domain-containing protein	-
AWN66_RS14715	3009385..3009861	-	477	WP_001186708.1	RadC family protein	-
AWN66_RS14720	3009877..3010362	-	486	WP_000206667.1	antirestriction protein	-
AWN66_RS14725	3010454..3011272	-	819	WP_001234592.1	DUF945 domain-containing protein	-
AWN66_RS14730	3011612..3012682	-	1071	WP_000102675.1	patatin-like phospholipase family protein	-
AWN66_RS14735	3012679..3013584	-	906	WP_000203561.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13993.03 Da
• Isoelectric Point: 8.2905

>T59822 WP_000854683.1 NZ_CP014004:c3008564-3008190 [Klebsiella pneumoniae subsp. pneumoniae]
MKTLPDTHIREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTQFADERVIELHIEAGISLCDAVNFLVEKYALVRTGQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T59822 NZ_CP014004:c3008564-3008190 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTACCCGACACACACATACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAACATTACGGCCTTACGCTGAATGACACACAGTTCGCGGATGAACGCGTGATTGAGCTGCATATTG
AGGCCGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGGCCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13354.17 Da
• Isoelectric Point: 6.6247

>AT59822 WP_024186744.1 NZ_CP014004:c3009016-3008654 [Klebsiella pneumoniae subsp. pneumoniae]
VSDTLPGTTHPDNNRPWWGLPCTVMPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGEL
NPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 363 bp

>AT59822 NZ_CP014004:c3009016-3008654 [Klebsiella pneumoniae subsp. pneumoniae]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCCCTG
TTTCGGCGCCCGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGTTCAGCG
ACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTG
AATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGTTGTGGTTA
CGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA