Detailed information of TA system TA059809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 40131..40711
• Replicon: plasmid unnamed5
• Accession: NZ_CP014003
• Organism: Synechococcus sp. PCC 73109

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AWQ23_RS15075
• Protein ID: WP_157072259.1
• Coordinates: 40131..40457 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B1U2U2
• Locus tag: AWQ23_RS15080
• Protein ID: WP_030008115.1
• Coordinates: 40469..40711 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ23_RS15055	35438..38023	+	2586	WP_062437154.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
AWQ23_RS15060	38149..38514	+	366	WP_062437162.1	VOC family protein	-
AWQ23_RS15065	38574..39467	+	894	WP_062436973.1	ABC transporter substrate-binding protein	-
AWQ23_RS15070	39532..40068	+	537	WP_062436975.1	hypothetical protein	-
AWQ23_RS15075	40131..40457	-	327	WP_157072259.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWQ23_RS15080	40469..40711	-	243	WP_030008115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWQ23_RS15085	40816..41631	-	816	WP_062436979.1	ABC transporter ATP-binding protein	-
AWQ23_RS15090	41635..42672	-	1038	WP_062436981.1	iron ABC transporter permease	-
AWQ23_RS15095	42672..43637	-	966	WP_062437163.1	iron ABC transporter permease	-
AWQ23_RS15100	43756..44661	-	906	WP_062436983.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..163688	163688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12193.33 Da
• Isoelectric Point: 8.5043

>T59809 WP_157072259.1 NZ_CP014003:c40457-40131 [Synechococcus sp. PCC 73109]
MVPKRGDIIFLELNPRTGSEQSGYRPAIVISPEEYNRISNLTLICPITSKEKGWVFEVKLPPNLKTQGVILVDQIRAVDC
RARKAKIVEQLPLDTLNEILARLEPLLT

Nucleotide

• Length: 327 bp

>T59809 NZ_CP014003:c40457-40131 [Synechococcus sp. PCC 73109]
ATGGTTCCTAAGCGGGGAGATATTATTTTTCTCGAACTGAATCCCCGCACAGGTAGTGAACAATCAGGTTATCGTCCGGC
GATCGTGATTTCCCCTGAGGAATATAATCGTATTTCTAATCTGACCCTAATCTGTCCAATCACGAGTAAAGAAAAAGGCT
GGGTGTTTGAGGTGAAACTTCCGCCTAATCTCAAAACCCAAGGCGTCATTCTGGTCGATCAAATCCGGGCGGTGGACTGT
CGAGCCAGGAAAGCAAAAATTGTGGAACAACTTCCCCTTGATACTCTTAATGAAATACTCGCGAGGTTAGAGCCTCTTTT
GACTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9119.28 Da
• Isoelectric Point: 4.5125

>AT59809 WP_030008115.1 NZ_CP014003:c40711-40469 [Synechococcus sp. PCC 73109]
MITQKITKWGNSFGIRLPQSMLQQCGWQEGEQVTISMENNQIILSLARPKYSLAELLKDAKPEAQHSEVDWGEAQGEESW

Nucleotide

• Length: 243 bp

>AT59809 NZ_CP014003:c40711-40469 [Synechococcus sp. PCC 73109]
ATGATCACCCAAAAAATCACGAAATGGGGCAACTCTTTTGGTATTCGCTTGCCTCAATCCATGCTCCAGCAATGCGGTTG
GCAGGAAGGGGAACAAGTGACGATATCAATGGAAAACAACCAAATTATTTTGAGCCTTGCTCGACCAAAATATAGCCTTG
CGGAATTACTCAAGGATGCAAAACCTGAAGCACAGCATTCTGAAGTGGATTGGGGGGAAGCCCAAGGAGAAGAGTCTTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1B1U2U2