59805

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 38446..39583 Replicon plasmid unnamed2
Accession NZ_CP013996
Organism Enterococcus faecium strain 6E6

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag AWJ25_RS16965 Protein ID WP_001284311.1
Coordinates 38720..39583 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag AWJ25_RS16960 Protein ID WP_000301765.1
Coordinates 38446..38718 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ25_RS16930 34266..34562 + 297 WP_000053907.1 replication control protein PrgN -
AWJ25_RS16935 34586..35545 - 960 WP_002287225.1 IS30 family transposase -
AWJ25_RS16940 35728..36579 + 852 WP_002287227.1 hypothetical protein -
AWJ25_RS16945 36600..36883 + 284 Protein_40 hypothetical protein -
AWJ25_RS16950 37245..37925 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
AWJ25_RS18355 37979..38122 + 144 Protein_42 chromosome partitioning protein ParA -
AWJ25_RS16955 38220..38429 + 210 WP_000527318.1 peptide-binding protein -
AWJ25_RS16960 38446..38718 + 273 WP_000301765.1 antitoxin Antitoxin
AWJ25_RS16965 38720..39583 + 864 WP_001284311.1 toxin zeta Toxin
AWJ25_RS16970 39846..40583 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
AWJ25_RS16975 40708..40791 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
AWJ25_RS18190 40840..40923 - 84 Protein_48 peptide-binding protein -
AWJ25_RS16980 41333..42127 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
AWJ25_RS18195 42220..42471 - 252 Protein_50 GNAT family N-acetyltransferase -
AWJ25_RS16985 42716..43999 + 1284 WP_059355968.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III - 1..49420 49420
- inside IScluster/Tn erm(B) / aph(3')-III - 28468..49292 20824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T59805 WP_001284311.1 NZ_CP013996:38720-39583 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T59805 NZ_CP013996:38720-39583 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT59805 WP_000301765.1 NZ_CP013996:38446-38718 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT59805 NZ_CP013996:38446-38718 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References