Detailed information of TA system TA059803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 310493..311100
• Replicon: plasmid unnamed1
• Accession: NZ_CP013995
• Organism: Enterococcus faecium strain 6E6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: AWJ25_RS16375
• Protein ID: WP_002321032.1
• Coordinates: 310493..310843 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: AWJ25_RS16380
• Protein ID: WP_002287514.1
• Coordinates: 310837..311100 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWJ25_RS16345	306072..306422	-	351	WP_002303114.1	hypothetical protein	-
AWJ25_RS16350	306415..307740	-	1326	WP_002303115.1	Y-family DNA polymerase	-
AWJ25_RS16355	308111..308695	-	585	WP_002299575.1	recombinase family protein	-
AWJ25_RS16360	308906..309109	-	204	WP_002299573.1	hypothetical protein	-
AWJ25_RS16365	309119..309541	-	423	WP_002288787.1	hypothetical protein	-
AWJ25_RS16370	309855..310469	+	615	WP_059355961.1	recombinase family protein	-
AWJ25_RS16375	310493..310843	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWJ25_RS16380	310837..311100	-	264	WP_002287514.1	hypothetical protein	Antitoxin
AWJ25_RS16385	311441..311620	-	180	WP_002305761.1	hypothetical protein	-
AWJ25_RS16395	312001..312708	-	708	WP_002353391.1	hypothetical protein	-
AWJ25_RS16400	312701..313138	-	438	WP_002296237.1	hypothetical protein	-
AWJ25_RS16405	313153..313404	-	252	WP_002296238.1	hypothetical protein	-
AWJ25_RS16410	313404..313610	-	207	WP_002296239.1	hypothetical protein	-
AWJ25_RS16415	314051..314311	-	261	WP_002296240.1	hypothetical protein	-
AWJ25_RS16420	314677..315162	-	486	WP_002296241.1	single-stranded DNA-binding protein	-
AWJ25_RS16425	315165..316064	-	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..381524	381524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T59803 WP_002321032.1 NZ_CP013995:c310843-310493 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T59803 NZ_CP013995:c310843-310493 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT59803 WP_002287514.1 NZ_CP013995:c311100-310837 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT59803 NZ_CP013995:c311100-310837 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0