Detailed information of TA system TA059802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 241591..242110
• Replicon: plasmid unnamed1
• Accession: NZ_CP013995
• Organism: Enterococcus faecium strain 6E6

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: AWJ25_RS15990
• Protein ID: WP_000588503.1
• Coordinates: 241591..241848 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: AWJ25_RS15995
• Protein ID: WP_000388479.1
• Coordinates: 241841..242110 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWJ25_RS15960	236814..237494	+	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
AWJ25_RS15965	237683..238267	-	585	WP_002299575.1	recombinase family protein	-
AWJ25_RS15970	238478..238681	-	204	WP_002299573.1	hypothetical protein	-
AWJ25_RS15975	238691..239113	-	423	WP_002303116.1	hypothetical protein	-
AWJ25_RS15980	239351..239965	+	615	WP_001261742.1	recombinase family protein	-
AWJ25_RS15985	240130..240954	-	825	WP_001809248.1	hypothetical protein	-
AWJ25_RS18610	240989..241132	-	144	WP_164857621.1	hypothetical protein	-
AWJ25_RS15990	241591..241848	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
AWJ25_RS15995	241841..242110	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AWJ25_RS16000	242241..242420	-	180	WP_001120991.1	hypothetical protein	-
AWJ25_RS16010	242801..243508	-	708	WP_002303117.1	hypothetical protein	-
AWJ25_RS16015	243501..243938	-	438	WP_002300851.1	hypothetical protein	-
AWJ25_RS16020	243953..244204	-	252	WP_002296238.1	hypothetical protein	-
AWJ25_RS16025	244204..244410	-	207	WP_002347218.1	hypothetical protein	-
AWJ25_RS16030	245011..246306	+	1296	WP_002297185.1	ISL3-like element ISEfa5 family transposase	-
AWJ25_RS16035	246366..246629	-	264	WP_059355952.1	hypothetical protein	-
AWJ25_RS16040	246704..246907	+	204	WP_002316074.1	putative holin-like toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..381524	381524
	-	flank	IS/Tn	-	-	245011..246306	1295

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T59802 WP_000588503.1 NZ_CP013995:c241848-241591 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T59802 NZ_CP013995:c241848-241591 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT59802 WP_000388479.1 NZ_CP013995:c242110-241841 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT59802 NZ_CP013995:c242110-241841 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7