Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2258346..2258917 Replicon chromosome
Accession NZ_CP013994
Organism Enterococcus faecium strain 6E6

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag AWJ25_RS11370 Protein ID WP_002286801.1
Coordinates 2258346..2258687 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag AWJ25_RS11375 Protein ID WP_002323011.1
Coordinates 2258687..2258917 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ25_RS11345 2253556..2254890 - 1335 WP_002303373.1 ABC transporter substrate-binding protein -
AWJ25_RS11350 2255098..2255928 + 831 WP_002286789.1 manganese catalase family protein -
AWJ25_RS17680 2256172..2256454 - 283 Protein_2218 transposase -
AWJ25_RS11360 2256612..2256806 - 195 WP_002297028.1 hypothetical protein -
AWJ25_RS11365 2257311..2257496 - 186 WP_002304207.1 hypothetical protein -
AWJ25_RS11370 2258346..2258687 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AWJ25_RS11375 2258687..2258917 - 231 WP_002323011.1 hypothetical protein Antitoxin
AWJ25_RS11380 2259204..2259542 + 339 WP_002286804.1 hypothetical protein -
AWJ25_RS11385 2259747..2259986 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
AWJ25_RS11390 2259962..2260309 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
AWJ25_RS11395 2261041..2261622 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
AWJ25_RS11400 2261805..2262431 - 627 WP_002286816.1 cysteine hydrolase -
AWJ25_RS11405 2262453..2263784 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T59801 WP_002286801.1 NZ_CP013994:c2258687-2258346 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T59801 NZ_CP013994:c2258687-2258346 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT59801 WP_002323011.1 NZ_CP013994:c2258917-2258687 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT59801 NZ_CP013994:c2258917-2258687 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References