Detailed information of TA system TA059798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5034858..5035444
• Replicon: chromosome
• Accession: NZ_CP013993
• Organism: Pseudomonas aeruginosa DHS01 strain DH01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: DPADHS01_RS23855
• Protein ID: WP_003120987.1
• Coordinates: 5035145..5035444 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DPADHS01_RS23850
• Protein ID: WP_003448662.1
• Coordinates: 5034858..5035148 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPADHS01_RS23830	5030009..5030218	+	210	WP_003105733.1	cold-shock protein	-
DPADHS01_RS23835	5030440..5032329	+	1890	WP_016851610.1	hypothetical protein	-
DPADHS01_RS23840	5032326..5034302	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
DPADHS01_RS23845	5034443..5034787	+	345	WP_016851612.1	hypothetical protein	-
DPADHS01_RS23850	5034858..5035148	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
DPADHS01_RS23855	5035145..5035444	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DPADHS01_RS23860	5035646..5036770	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
DPADHS01_RS23865	5036770..5038479	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
DPADHS01_RS23870	5038483..5039808	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
DPADHS01_RS23875	5039798..5040331	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5007879..5102356	94477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T59798 WP_003120987.1 NZ_CP013993:c5035444-5035145 [Pseudomonas aeruginosa DHS01]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T59798 NZ_CP013993:c5035444-5035145 [Pseudomonas aeruginosa DHS01]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT59798 WP_003448662.1 NZ_CP013993:c5035148-5034858 [Pseudomonas aeruginosa DHS01]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT59798 NZ_CP013993:c5035148-5034858 [Pseudomonas aeruginosa DHS01]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73