Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3691262..3691881 Replicon chromosome
Accession NZ_CP013990
Organism Leclercia adecarboxylata strain USDA-ARS-USMARC-60222

Toxin (Protein)


Gene name Hha Uniprot ID A0A345CDT0
Locus tag APT61_RS17175 Protein ID WP_032616864.1
Coordinates 3691663..3691881 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3E1ZZ82
Locus tag APT61_RS17170 Protein ID WP_032616865.1
Coordinates 3691262..3691654 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APT61_RS17160 3686415..3687608 + 1194 WP_032616867.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
APT61_RS17165 3687631..3690771 + 3141 WP_059308599.1 multidrug efflux RND transporter permease subunit AcrB -
APT61_RS17170 3691262..3691654 + 393 WP_032616865.1 Hha toxicity modulator TomB Antitoxin
APT61_RS17175 3691663..3691881 + 219 WP_032616864.1 hemolysin expression modulator Hha Toxin
APT61_RS17180 3692153..3692617 + 465 WP_032616863.1 membrane protein -
APT61_RS23005 3692677..3692817 - 141 WP_032616862.1 type B 50S ribosomal protein L36 -
APT61_RS17185 3693083..3694651 + 1569 WP_059308600.1 EAL domain-containing protein -
APT61_RS17190 3694684..3695037 - 354 WP_059308601.1 DUF1428 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T59788 WP_032616864.1 NZ_CP013990:3691663-3691881 [Leclercia adecarboxylata]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Download         Length: 219 bp

>T59788 NZ_CP013990:3691663-3691881 [Leclercia adecarboxylata]
ATGTCTGATAAACCCTTAACCAAGACAGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATTGACACCCTTGAACGTGT
GATTGAGAAAAATAAATACGAGCTTTCTGATAATGAGTTAGCAGTATTCTATTCTGCAGCCGATCATCGTCTGGCTGAAC
TCACTATGAATAAGCTGTATGATAAAATCCCGACAGCCGTGTGGAAGTTTGTACGCTGA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 15093.98 Da        Isoelectric Point: 4.8941

>AT59788 WP_032616865.1 NZ_CP013990:3691262-3691654 [Leclercia adecarboxylata]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASKANPLASPVKIITNN

Download         Length: 393 bp

>AT59788 NZ_CP013990:3691262-3691654 [Leclercia adecarboxylata]
ATGGACGAATACTCGCCTAAAAGGCAAGATATTGCACAGTTGAAATTCCTTTGTGAATCCCTGTACCATGACTGCCTTTC
CAATCTCGATGAAAGCAACCATGGCTGGGTCAATGACCCTACTTCTGCCATTAATTTGCAGTTAAACGAGTTGATTGAGC
ATATAGCAACCTTCGCGCTTAATTATAAAATTAAGTATAACGAAGATAATAAGTTGATTGAGCAAATTGACGAATACCTG
GATGACACTTTTATGTTATTCAGCAGCTACGGCATTAATGCACAGGATTTGCAAAAATGGCGTAAATCGGGCAATCGGCT
TTTTCGGTGTTTTGTCAATGCAAGCAAGGCTAACCCGTTAGCTTCGCCAGTTAAAATTATTACAAACAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A345CDT0

Antitoxin

Source ID Structure
AlphaFold DB A0A3E1ZZ82

References