Detailed information of TA system TA059786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2367199..2367773
• Replicon: chromosome
• Accession: NZ_CP013990
• Organism: Leclercia adecarboxylata strain USDA-ARS-USMARC-60222

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: APT61_RS10980
• Protein ID: WP_059307894.1
• Coordinates: 2367199..2367525 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6G7TED5
• Locus tag: APT61_RS10985
• Protein ID: WP_059307895.1
• Coordinates: 2367525..2367773 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT61_RS10960	2362777..2363469	+	693	WP_032618178.1	ABC transporter ATP-binding protein	-
APT61_RS10965	2363466..2364434	+	969	WP_059307891.1	ABC transporter substrate-binding protein	-
APT61_RS10970	2364473..2366128	+	1656	WP_156424697.1	iron ABC transporter permease	-
APT61_RS10975	2366325..2367170	+	846	WP_059307893.1	N-hydroxyarylamine O-acetyltransferase	-
APT61_RS10980	2367199..2367525	-	327	WP_059307894.1	endoribonuclease MazF	Toxin
APT61_RS10985	2367525..2367773	-	249	WP_059307895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
APT61_RS10990	2367953..2368633	-	681	WP_059307896.1	respiratory nitrate reductase subunit gamma	-
APT61_RS10995	2368630..2369325	-	696	WP_059307897.1	nitrate reductase molybdenum cofactor assembly chaperone	-
APT61_RS11000	2369325..2370869	-	1545	WP_059307898.1	nitrate reductase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11875.64 Da
• Isoelectric Point: 7.8831

>T59786 WP_059307894.1 NZ_CP013990:c2367525-2367199 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVISPATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T59786 NZ_CP013990:c2367525-2367199 [Leclercia adecarboxylata]
ATGGACTACATCCCGGAAGCGGGCGATATCGTCTGGCTGGATTTTGATCCGCAGTCAGGTCATGAACAGGCAGGCCATCG
CCCTGCGCTGGTTATTTCACCCGCCACTTACAATGGACGCACCGGTCTGATGCTCTGCTGTCCAGTCACGTCGCAAATCA
AAAACTACCCCTTCGAAGTTGTCATTAGCGGAGCACGCCGCAATGTCGCCCTCGCCGATCAGATCAAAAGTCTGGACTGG
CGCGCGCGTCACGCAGTGAAAAAGGGAACGGCAACTGCCGAAGAACTGGATGCCGTGAGAATGCGGGCGAAGGTTTTGAT
CGGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9137.39 Da
• Isoelectric Point: 4.4777

>AT59786 WP_059307895.1 NZ_CP013990:c2367773-2367525 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT59786 NZ_CP013990:c2367773-2367525 [Leclercia adecarboxylata]
ATGCCGCAAGTCACCGTCAAAAAATGGGGTAACAGCCCTTCTGTCAGGCTGCCTGTCGCAGTTATGCGCGAGGCTGAATT
GCACGTTGACGATGTGGTGAACGTTAACGTAGACGAAGAAGGACGCATTATTTTGACACCCGTCAGGAAGGACGAACCGA
CGCTGGAATCGCTGCTCGCTGCGATCACCGACGACAATCTGCATCACGAAGTCACCTTTGGAGAACCGCAAGGTAAGGAG
CTGTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G7TED5