59774

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4830531..4831139 Replicon chromosome
Accession NZ_CP013989
Organism Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639

Toxin (Protein)


Gene name PfiT Uniprot ID A0A3S4N9Q9
Locus tag APT60_RS22250 Protein ID WP_031672658.1
Coordinates 4830531..4830878 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag APT60_RS30100 Protein ID WP_003114155.1
Coordinates 4830888..4831139 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APT60_RS22220 4825891..4826163 + 273 WP_003120794.1 cysteine-rich CWC family protein -
APT60_RS22225 4826163..4826855 + 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
APT60_RS22230 4826991..4828034 + 1044 WP_003110811.1 L,D-transpeptidase -
APT60_RS22235 4828114..4828851 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
APT60_RS22240 4829302..4830204 + 903 WP_003116518.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
APT60_RS22250 4830531..4830878 - 348 WP_031672658.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
APT60_RS30100 4830888..4831139 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
APT60_RS22255 4831353..4832336 - 984 WP_031672657.1 tyrosine-type recombinase/integrase -
APT60_RS22260 4832336..4833628 - 1293 WP_003123045.1 hypothetical protein -
APT60_RS22265 4833887..4835149 - 1263 WP_031672622.1 hypothetical protein -
APT60_RS22270 4835151..4835501 - 351 WP_003133726.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4830531..4852383 21852


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12898.69 Da        Isoelectric Point: 4.5143

>T59774 WP_031672658.1 NZ_CP013989:c4830878-4830531 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGAQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T59774 NZ_CP013989:c4830878-4830531 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGCACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT59774 WP_003114155.1 NZ_CP013989:c4831139-4830888 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT59774 NZ_CP013989:c4831139-4830888 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S4N9Q9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References