Detailed information of TA system TA059773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4468500..4469758
• Replicon: chromosome
• Accession: NZ_CP013989
• Organism: Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: APT60_RS20510
• Protein ID: WP_003163133.1
• Coordinates: 4468500..4469255 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: APT60_RS20515
• Protein ID: WP_003086329.1
• Coordinates: 4469390..4469758 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT60_RS20500	4463672..4466215	+	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-
APT60_RS20505	4466370..4467731	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
APT60_RS20510	4468500..4469255	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
APT60_RS20515	4469390..4469758	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
APT60_RS20520	4469831..4471117	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
APT60_RS20525	4471173..4472762	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
APT60_RS20530	4472841..4473317	+	477	WP_003140638.1	hypothetical protein	-
APT60_RS30650	4473320..4473595	-	276	WP_172826506.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T59773 WP_003163133.1 NZ_CP013989:c4469255-4468500 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T59773 NZ_CP013989:c4469255-4468500 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT59773 WP_003086329.1 NZ_CP013989:c4469758-4469390 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT59773 NZ_CP013989:c4469758-4469390 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7