Detailed information of TA system TA059770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 172625..173130
• Replicon: chromosome
• Accession: NZ_CP013989
• Organism: Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: APT60_RS00770
• Protein ID: WP_003083773.1
• Coordinates: 172625..172906 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: APT60_RS00775
• Protein ID: WP_003083775.1
• Coordinates: 172903..173130 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT60_RS00745	167876..169225	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
APT60_RS00750	169274..169960	+	687	WP_003083762.1	FadR family transcriptional regulator	-
APT60_RS00755	170061..170795	+	735	WP_059349409.1	GntR family transcriptional regulator	-
APT60_RS00760	170999..171385	+	387	WP_003083767.1	aegerolysin family protein	-
APT60_RS00765	171417..172325	-	909	WP_003083769.1	LysR family transcriptional regulator	-
APT60_RS00770	172625..172906	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
APT60_RS00775	172903..173130	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
APT60_RS00780	173307..173927	-	621	WP_009314940.1	hypothetical protein	-
APT60_RS00785	174028..174528	+	501	WP_003112629.1	LEA type 2 family protein	-
APT60_RS00790	174601..174942	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
APT60_RS00795	175024..176451	-	1428	WP_003083784.1	GABA permease	-
APT60_RS00800	176620..178113	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T59770 WP_003083773.1 NZ_CP013989:c172906-172625 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T59770 NZ_CP013989:c172906-172625 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT59770 WP_003083775.1 NZ_CP013989:c173130-172903 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT59770 NZ_CP013989:c173130-172903 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9