Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58763..59499 Replicon plasmid unnamed
Accession NZ_CP013986
Organism Klebsiella variicola strain LMG 23571

Toxin (Protein)


Gene name tacT Uniprot ID A0A6M6A403
Locus tag AWN63_RS28110 Protein ID WP_023288256.1
Coordinates 59017..59499 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AWN63_RS28105 Protein ID WP_003026799.1
Coordinates 58763..59029 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN63_RS28075 54044..54262 + 219 WP_119867233.1 hypothetical protein -
AWN63_RS28080 54341..54667 + 327 WP_123797897.1 hypothetical protein -
AWN63_RS28085 54907..55164 + 258 WP_119867235.1 hypothetical protein -
AWN63_RS28090 55164..55730 + 567 WP_119867236.1 hypothetical protein -
AWN63_RS28095 55761..56252 + 492 WP_119867237.1 DNA-binding protein -
AWN63_RS28735 56276..56788 - 513 WP_123797898.1 hypothetical protein -
AWN63_RS28100 57411..58196 + 786 WP_123797899.1 hypothetical protein -
AWN63_RS28740 58359..58556 - 198 WP_123797900.1 hypothetical protein -
AWN63_RS28105 58763..59029 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AWN63_RS28110 59017..59499 + 483 WP_023288256.1 GNAT family N-acetyltransferase Toxin
AWN63_RS28115 59767..60330 + 564 WP_181446353.1 AraC family transcriptional regulator -
AWN63_RS28120 60473..60772 - 300 WP_101880805.1 antibiotic biosynthesis monooxygenase -
AWN63_RS28130 61802..63214 - 1413 WP_119867240.1 amino acid permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..163254 163254
- flank IS/Tn - - 61099..61200 101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17296.98 Da        Isoelectric Point: 8.7400

>T59769 WP_023288256.1 NZ_CP013986:59017-59499 [Klebsiella variicola]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T59769 NZ_CP013986:59017-59499 [Klebsiella variicola]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACAGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT59769 WP_003026799.1 NZ_CP013986:58763-59029 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT59769 NZ_CP013986:58763-59029 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M6A403

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References