Detailed information of TA system TA059768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 53259..53902
• Replicon: plasmid unnamed
• Accession: NZ_CP013986
• Organism: Klebsiella variicola strain LMG 23571

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AWN63_RS28070
• Protein ID: WP_119867232.1
• Coordinates: 53486..53902 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: AWN63_RS28065
• Protein ID: WP_119867231.1
• Coordinates: 53259..53489 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN63_RS28035	49004..49489	-	486	WP_119867226.1	TIR domain-containing protein	-
AWN63_RS28040	49501..50028	-	528	WP_119867227.1	toll/interleukin-1 receptor domain-containing protein	-
AWN63_RS28045	50582..50812	+	231	WP_119867228.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AWN63_RS28050	50809..51225	+	417	WP_119867229.1	type II toxin-antitoxin system VapC family toxin	-
AWN63_RS28060	51729..52739	-	1011	WP_123797896.1	hypothetical protein	-
AWN63_RS28065	53259..53489	+	231	WP_119867231.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWN63_RS28070	53486..53902	+	417	WP_119867232.1	type II toxin-antitoxin system VapC family toxin	Toxin
AWN63_RS28075	54044..54262	+	219	WP_119867233.1	hypothetical protein	-
AWN63_RS28080	54341..54667	+	327	WP_123797897.1	hypothetical protein	-
AWN63_RS28085	54907..55164	+	258	WP_119867235.1	hypothetical protein	-
AWN63_RS28090	55164..55730	+	567	WP_119867236.1	hypothetical protein	-
AWN63_RS28095	55761..56252	+	492	WP_119867237.1	DNA-binding protein	-
AWN63_RS28735	56276..56788	-	513	WP_123797898.1	hypothetical protein	-
AWN63_RS28100	57411..58196	+	786	WP_123797899.1	hypothetical protein	-
AWN63_RS28740	58359..58556	-	198	WP_123797900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..163254	163254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15038.58 Da
• Isoelectric Point: 9.2957

>T59768 WP_119867232.1 NZ_CP013986:53486-53902 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAMLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T59768 NZ_CP013986:53486-53902 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTACTGAAGCGCCT
GGAGCAGGCGATGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGCGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8663.71 Da
• Isoelectric Point: 4.4563

>AT59768 WP_119867231.1 NZ_CP013986:53259-53489 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLGRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT59768 NZ_CP013986:53259-53489 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
GCAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA