Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4906337..4906913 Replicon chromosome
Accession NZ_CP013985
Organism Klebsiella variicola strain LMG 23571

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag AWN63_RS24320 Protein ID WP_012542979.1
Coordinates 4906626..4906913 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag AWN63_RS24315 Protein ID WP_012542978.1
Coordinates 4906337..4906639 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN63_RS24290 4901745..4902809 - 1065 WP_008807596.1 DUF2955 domain-containing protein -
AWN63_RS24295 4902799..4903866 - 1068 WP_063106329.1 HlyD family secretion protein -
AWN63_RS24300 4903873..4904337 - 465 WP_100698682.1 MarR family transcriptional regulator -
AWN63_RS24305 4904503..4904667 - 165 WP_008807599.1 DUF1127 domain-containing protein -
AWN63_RS24310 4904845..4906257 + 1413 WP_012969021.1 PLP-dependent aminotransferase family protein -
AWN63_RS24315 4906337..4906639 - 303 WP_012542978.1 BrnA antitoxin family protein Antitoxin
AWN63_RS24320 4906626..4906913 - 288 WP_012542979.1 BrnT family toxin Toxin
AWN63_RS24325 4907081..4907500 - 420 WP_119867150.1 FosA family fosfomycin resistance glutathione transferase -
AWN63_RS24330 4907494..4908402 - 909 WP_012542980.1 LysR family transcriptional regulator -
AWN63_RS24335 4908489..4909271 + 783 WP_012542981.1 NAD(P)H-dependent oxidoreductase -
AWN63_RS24340 4909413..4909997 + 585 WP_022065974.1 TetR/AcrR family transcriptional regulator -
AWN63_RS24345 4910019..4910822 + 804 WP_022065975.1 winged helix-turn-helix domain-containing protein -
AWN63_RS24350 4910819..4911337 + 519 WP_004182811.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11245.77 Da        Isoelectric Point: 8.6574

>T59762 WP_012542979.1 NZ_CP013985:c4906913-4906626 [Klebsiella variicola]
MPMEFEWDANKALSNLRKHGVRFEEAVLVFDDPRHLSRQERFENGEYRWQTIGLVHGILVILVAHSVRFESGTEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T59762 NZ_CP013985:c4906913-4906626 [Klebsiella variicola]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCATTAAGTAATCTACGAAAACATGGAGTCCGGTTTGAAGAAGCGGT
ATTAGTCTTTGACGATCCGCGGCACCTTTCCCGCCAGGAGCGGTTTGAGAATGGTGAATATCGCTGGCAGACCATCGGTC
TTGTGCATGGGATCCTTGTTATCCTGGTGGCGCATTCGGTCCGTTTTGAAAGTGGAACTGAGGTTATTCGTATTATCAGC
GCCCGGAAGGCGGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11379.02 Da        Isoelectric Point: 10.1284

>AT59762 WP_012542978.1 NZ_CP013985:c4906639-4906337 [Klebsiella variicola]
MSMVKHKRDNTAALTAKRDAELKALASKADEDIDYSDIPATDDAQWSDAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT59762 NZ_CP013985:c4906639-4906337 [Klebsiella variicola]
ATGAGCATGGTTAAACATAAACGCGACAATACGGCGGCGCTTACCGCTAAGCGTGATGCCGAACTCAAAGCGCTAGCGAG
CAAAGCCGATGAGGATATCGATTACAGCGATATCCCCGCTACAGACGATGCGCAATGGTCAGATGCCGTTCGCGGCAAAT
TTTTTCGACCGCTAAAAACACAGGCTTCTGTGCGCATTGATGCCGATGTCATGGAGTGGCTCAAACGTCCAGGAAAAGGC
TATCAAACTCGACTGAATGCGATTTTAAGAGAAGCGATGATTCGCGATCTTAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References