Detailed information of TA system TA059747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1776769..1777298
• Replicon: chromosome
• Accession: NZ_CP013980
• Organism: Staphylococcus equorum strain KM1031

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S376
• Locus tag: AWC34_RS08550
• Protein ID: WP_002511418.1
• Coordinates: 1776769..1777131 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J9GY23
• Locus tag: AWC34_RS08555
• Protein ID: WP_002507108.1
• Coordinates: 1777128..1777298 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC34_RS08530	1773774..1774544	-	771	WP_002507114.1	RNA polymerase sigma factor SigB	-
AWC34_RS08535	1774519..1775001	-	483	WP_002507113.1	anti-sigma B factor RsbW	-
AWC34_RS08540	1775003..1775329	-	327	WP_002511417.1	anti-sigma factor antagonist	-
AWC34_RS08545	1775409..1776410	-	1002	WP_002507111.1	PP2C family protein-serine/threonine phosphatase	-
AWC34_RS08550	1776769..1777131	-	363	WP_002511418.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWC34_RS08555	1777128..1777298	-	171	WP_002507108.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AWC34_RS08560	1777386..1778534	-	1149	WP_021339950.1	alanine racemase	-
AWC34_RS08565	1778658..1779008	-	351	WP_021339949.1	holo-ACP synthase	-
AWC34_RS08570	1779041..1779577	-	537	WP_002507105.1	PH domain-containing protein	-
AWC34_RS08575	1779570..1781075	-	1506	WP_065367469.1	PH domain-containing protein	-
AWC34_RS08580	1781068..1781547	-	480	WP_021339947.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13432.59 Da
• Isoelectric Point: 10.2178

>T59747 WP_002511418.1 NZ_CP013980:c1777131-1776769 [Staphylococcus equorum]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSESKMKEVNVAIDISLGLHNVRNSKS

Nucleotide

• Length: 363 bp

>T59747 NZ_CP013980:c1777131-1776769 [Staphylococcus equorum]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATCATACAGAATGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACAGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAGCTTGATAAAGATTCTGTAATACTGCTTGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACATACCTTTCTGAAAGTAAAATGAAAGAAGTTAATGTTGCCATAGA
TATAAGCTTAGGATTACATAATGTTAGAAATAGTAAATCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT59747 WP_002507108.1 NZ_CP013980:c1777298-1777128 [Staphylococcus equorum]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYLSSKNNNE

Nucleotide

• Length: 171 bp

>AT59747 NZ_CP013980:c1777298-1777128 [Staphylococcus equorum]
ATGGCAAGTTTTAATCAAAGCAGAAACCATAGTATTGAACAACTATTAAAAGAAGGCTATTCACAAATGGCCGATTTAAA
TCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACCTCAGCTCTAAGAATA
ACAATGAATGA

Structures

Toxin

• AlphaFold DB: K7S376

Antitoxin

• AlphaFold DB: J9GY23