Detailed information of TA system TA059739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 22890..23496
• Replicon: plasmid pPSCRG02-2
• Accession: NZ_CP013977
• Organism: Piscirickettsia salmonis strain CGR02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AWJ11_RS16760
• Protein ID: WP_027242955.1
• Coordinates: 23236..23496 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: AWJ11_RS16755
• Protein ID: WP_016211078.1
• Coordinates: 22890..23243 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWJ11_RS20665	18652..18798	+	147	WP_169834793.1	hypothetical protein	-
AWJ11_RS18760	19030..20004	-	975	WP_036771330.1	IS30 family transposase	-
AWJ11_RS16730	20102..20449	-	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
AWJ11_RS16735	20442..20696	-	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
AWJ11_RS16740	20841..21206	-	366	WP_027242954.1	hypothetical protein	-
AWJ11_RS16745	21403..21630	-	228	Protein_31	hypothetical protein	-
AWJ11_RS18765	21739..22713	-	975	WP_069971681.1	IS30 family transposase	-
AWJ11_RS16755	22890..23243	-	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AWJ11_RS16760	23236..23496	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AWJ11_RS16765	23727..24317	+	591	WP_016212329.1	site-specific integrase	-
AWJ11_RS16770	24383..24739	-	357	WP_075278739.1	transposase	-
AWJ11_RS18770	24853..25827	+	975	WP_036771330.1	IS30 family transposase	-
AWJ11_RS16780	25824..26267	-	444	WP_178099494.1	transposase	-
AWJ11_RS20670	26472..26747	-	276	WP_171824216.1	hypothetical protein	-
AWJ11_RS16790	27195..28178	-	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33498	33498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59739 WP_027242955.1 NZ_CP013977:c23496-23236 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59739 NZ_CP013977:c23496-23236 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT59739 WP_016211078.1 NZ_CP013977:c23243-22890 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT59739 NZ_CP013977:c23243-22890 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4