Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 31839..32482 Replicon plasmid pPSCRG02-1
Accession NZ_CP013976
Organism Piscirickettsia salmonis strain CGR02

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L6TI66
Locus tag AWJ11_RS16105 Protein ID WP_027242931.1
Coordinates 32075..32482 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L6TI10
Locus tag AWJ11_RS16100 Protein ID WP_027242932.1
Coordinates 31839..32066 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ11_RS16075 27687..28094 - 408 WP_036817201.1 AAA family ATPase -
AWJ11_RS16080 28202..29245 - 1044 WP_017375960.1 NAD(P)-dependent alcohol dehydrogenase -
AWJ11_RS16085 29547..29780 + 234 WP_017375959.1 type II toxin-antitoxin system RelE/ParE family toxin -
AWJ11_RS19940 29918..30067 - 150 WP_196214251.1 hypothetical protein -
AWJ11_RS16090 30133..30462 + 330 WP_162038692.1 HigA family addiction module antidote protein -
AWJ11_RS16095 31341..31706 + 366 WP_026063496.1 hypothetical protein -
AWJ11_RS16100 31839..32066 + 228 WP_027242932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AWJ11_RS16105 32075..32482 + 408 WP_027242931.1 DUF4411 family protein Toxin
AWJ11_RS16110 32628..34010 + 1383 WP_027242930.1 hypothetical protein -
AWJ11_RS19945 34200..34403 + 204 WP_017375952.1 hypothetical protein -
AWJ11_RS16120 34599..34982 + 384 WP_027242929.1 HNH endonuclease -
AWJ11_RS16125 35069..35551 + 483 WP_075278733.1 P27 family phage terminase small subunit -
AWJ11_RS16130 35554..36885 + 1332 WP_048876205.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..115926 115926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-127)

Antitoxin

SpoVT-AbrB domain

(7-41)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15562.93 Da        Isoelectric Point: 7.4168

>T59737 WP_027242931.1 NZ_CP013976:32075-32482 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLDVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVDHLELEDWAS

Download         Length: 408 bp

>T59737 NZ_CP013976:32075-32482 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAACCTCGCTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCGATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGCAGTCAAC
ACCAGCGAAAAAATCAGGAAGCAATTAATACCTTGGTGAATAAGCTCGATGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCACATGC
GCTTTCAGAAAAGGCTGTTTTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGATCATTTAGAGCTTGAAGACTGGG
CAAGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8948.07 Da        Isoelectric Point: 4.5700

>AT59737 WP_027242932.1 NZ_CP013976:31839-32066 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Download         Length: 228 bp

>AT59737 NZ_CP013976:31839-32066 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCTCAAGCCGTTCGATTACCGAAAGAAATGCGCCTTGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAGGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6TI66

Antitoxin

Source ID Structure
AlphaFold DB A0A1L6TI10

References