Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2289762..2290276 Replicon chromosome
Accession NZ_CP013953
Organism Staphylococcus aureus strain NCCP14558 isolate sequencing

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag ASL16_RS11680 Protein ID WP_000581792.1
Coordinates 2289762..2289971 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag ASL16_RS11685 Protein ID WP_000138932.1
Coordinates 2289983..2290276 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASL16_RS11660 2285270..2286628 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
ASL16_RS11665 2286643..2287713 - 1071 WP_042109557.1 D-alanine--D-alanine ligase -
ASL16_RS11670 2288031..2289233 + 1203 WP_001109939.1 rod shape-determining protein RodA -
ASL16_RS11675 2289497..2289634 - 138 WP_000828354.1 hypothetical protein -
ASL16_RS11680 2289762..2289971 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
ASL16_RS11685 2289983..2290276 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
ASL16_RS11690 2290442..2291926 + 1485 WP_000571549.1 cardiolipin synthase -
ASL16_RS11695 2291954..2292601 + 648 WP_001187630.1 HD domain-containing protein -
ASL16_RS11705 2293514..2294386 - 873 WP_000725802.1 membrane protein insertase YidC -
ASL16_RS11710 2294472..2295113 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T59685 WP_000581792.1 NZ_CP013953:c2289971-2289762 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T59685 NZ_CP013953:c2289971-2289762 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT59685 WP_000138932.1 NZ_CP013953:c2290276-2289983 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT59685 NZ_CP013953:c2290276-2289983 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References