Detailed information of TA system TA059684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2268503..2269032
• Replicon: chromosome
• Accession: NZ_CP013953
• Organism: Staphylococcus aureus strain NCCP14558 isolate sequencing

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ASL16_RS11585
• Protein ID: WP_000621175.1
• Coordinates: 2268503..2268865 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ASL16_RS11590
• Protein ID: WP_000948331.1
• Coordinates: 2268862..2269032 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASL16_RS11560	2265481..2266251	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
ASL16_RS11565	2266226..2266705	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
ASL16_RS11570	2266707..2267033	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ASL16_RS11575	2267152..2268153	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ASL16_RS11585	2268503..2268865	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ASL16_RS11590	2268862..2269032	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ASL16_RS11595	2269117..2270265	-	1149	WP_001281154.1	alanine racemase	-
ASL16_RS11600	2270331..2270690	-	360	WP_000581197.1	holo-ACP synthase	-
ASL16_RS11605	2270694..2271185	-	492	WP_001205912.1	PH domain-containing protein	-
ASL16_RS11610	2271172..2272755	-	1584	WP_001294626.1	PH domain-containing protein	-
ASL16_RS11615	2272748..2273227	-	480	WP_001287087.1	hypothetical protein	-
ASL16_RS11620	2273435..2273995	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T59684 WP_000621175.1 NZ_CP013953:c2268865-2268503 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T59684 NZ_CP013953:c2268865-2268503 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT59684 WP_000948331.1 NZ_CP013953:c2269032-2268862 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT59684 NZ_CP013953:c2269032-2268862 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1