Detailed information of TA system TA059651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1354914..1355728
• Replicon: chromosome
• Accession: NZ_CP013952
• Organism: Escherichia coli strain HST04

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: AWH44_RS07115
• Protein ID: WP_001054376.1
• Coordinates: 1354914..1355171 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: AWH44_RS07120
• Protein ID: WP_001309181.1
• Coordinates: 1355183..1355728 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWH44_RS07080	1350202..1351308	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
AWH44_RS07085	1351373..1352353	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AWH44_RS07095	1352936..1354176	-	1241	Protein_1287	helicase YjhR	-
AWH44_RS23740	1354292..1354537	+	246	Protein_1288	GNAT family N-acetyltransferase	-
AWH44_RS07115	1354914..1355171	+	258	WP_001054376.1	hypothetical protein	Toxin
AWH44_RS07120	1355183..1355728	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
AWH44_RS07125	1355784..1356530	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
AWH44_RS07130	1356699..1356917	+	219	Protein_1292	hypothetical protein	-
AWH44_RS24180	1356955..1357071	+	117	Protein_1293	VOC family protein	-
AWH44_RS07140	1357316..1358437	+	1122	WP_000010829.1	M42 family metallopeptidase	-
AWH44_RS07145	1358434..1358712	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
AWH44_RS07150	1358724..1360037	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	1343924..1363952	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T59651 WP_001054376.1 NZ_CP013952:1354914-1355171 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T59651 NZ_CP013952:1354914-1355171 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT59651 WP_001309181.1 NZ_CP013952:1355183-1355728 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT59651 NZ_CP013952:1355183-1355728 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8