Detailed information of TA system TA059645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1767387..1768024
• Replicon: chromosome
• Accession: NZ_CP013950
• Organism: Bacillus sp. SDLI1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AUL54_RS08365
• Protein ID: WP_003156187.1
• Coordinates: 1767387..1767737 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AUL54_RS08370
• Protein ID: WP_003156188.1
• Coordinates: 1767743..1768024 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUL54_RS08325	1762424..1763026	-	603	WP_060963542.1	SpoIIE family protein phosphatase	-
AUL54_RS08330	1763026..1763814	-	789	WP_060963543.1	RNA polymerase sigma factor SigB	-
AUL54_RS08335	1763780..1764262	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
AUL54_RS08340	1764259..1764588	-	330	WP_016937169.1	anti-sigma factor antagonist	-
AUL54_RS08345	1764652..1765659	-	1008	WP_016937170.1	PP2C family protein-serine/threonine phosphatase	-
AUL54_RS08350	1765671..1766072	-	402	WP_003156178.1	anti-sigma regulatory factor	-
AUL54_RS08355	1766075..1766440	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AUL54_RS08360	1766445..1767266	-	822	WP_060963544.1	STAS domain-containing protein	-
AUL54_RS08365	1767387..1767737	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AUL54_RS08370	1767743..1768024	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AUL54_RS08375	1768144..1769313	-	1170	WP_016937171.1	alanine racemase	-
AUL54_RS08380	1769429..1770436	-	1008	WP_016937172.1	outer membrane lipoprotein carrier protein LolA	-
AUL54_RS08385	1770601..1770966	-	366	WP_060963545.1	holo-ACP synthase	-
AUL54_RS08390	1771059..1771658	+	600	WP_016937174.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T59645 WP_003156187.1 NZ_CP013950:c1767737-1767387 [Bacillus sp. SDLI1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T59645 NZ_CP013950:c1767737-1767387 [Bacillus sp. SDLI1]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT59645 WP_003156188.1 NZ_CP013950:c1768024-1767743 [Bacillus sp. SDLI1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT59645 NZ_CP013950:c1768024-1767743 [Bacillus sp. SDLI1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCACAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAACATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5