Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 160738..161381 Replicon plasmid pPsCRG01-1
Accession NZ_CP013946
Organism Piscirickettsia salmonis strain PSCGR01

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L6TI66
Locus tag AWE47_RS16990 Protein ID WP_027242931.1
Coordinates 160974..161381 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L6TI10
Locus tag AWE47_RS16985 Protein ID WP_027242932.1
Coordinates 160738..160965 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWE47_RS16960 156669..157235 - 567 WP_017375966.1 HAMP domain-containing histidine kinase -
AWE47_RS20510 157380..157898 - 519 WP_155048090.1 hypothetical protein -
AWE47_RS16970 157849..158274 - 426 WP_017375964.1 hypothetical protein -
AWE47_RS16975 158545..159540 - 996 WP_036817204.1 hypothetical protein -
AWE47_RS16980 160240..160605 + 366 WP_026063496.1 hypothetical protein -
AWE47_RS16985 160738..160965 + 228 WP_027242932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AWE47_RS16990 160974..161381 + 408 WP_027242931.1 DUF4411 family protein Toxin
AWE47_RS16995 161527..162909 + 1383 WP_027242930.1 hypothetical protein -
AWE47_RS20515 163099..163302 + 204 WP_017375952.1 hypothetical protein -
AWE47_RS17005 163498..163881 + 384 WP_027242929.1 HNH endonuclease -
AWE47_RS17010 163968..164450 + 483 WP_075278733.1 P27 family phage terminase small subunit -
AWE47_RS17015 164453..165784 + 1332 WP_048876205.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..179831 179831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-127)

Antitoxin

SpoVT-AbrB domain

(7-41)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15562.93 Da        Isoelectric Point: 7.4168

>T59639 WP_027242931.1 NZ_CP013946:160974-161381 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLDVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVDHLELEDWAS

Download         Length: 408 bp

>T59639 NZ_CP013946:160974-161381 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAACCTCGCTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCGATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGCAGTCAAC
ACCAGCGAAAAAATCAGGAAGCAATTAATACCTTGGTGAATAAGCTCGATGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCACATGC
GCTTTCAGAAAAGGCTGTTTTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGATCATTTAGAGCTTGAAGACTGGG
CAAGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8948.07 Da        Isoelectric Point: 4.5700

>AT59639 WP_027242932.1 NZ_CP013946:160738-160965 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Download         Length: 228 bp

>AT59639 NZ_CP013946:160738-160965 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCTCAAGCCGTTCGATTACCGAAAGAAATGCGCCTTGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAGGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6TI66

Antitoxin

Source ID Structure
AlphaFold DB A0A1L6TI10

References