Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 15551..16194 Replicon plasmid pCMA2
Accession NZ_CP013942
Organism Cronobacter malonaticus LMG 23826

Toxin (Protein)


Gene name vagD Uniprot ID V5U5J6
Locus tag AFK66_RS20665 Protein ID WP_007778986.1
Coordinates 15551..15967 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V5U780
Locus tag AFK66_RS20670 Protein ID WP_007778988.1
Coordinates 15964..16194 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK66_RS21945 10843..11166 - 324 WP_012815896.1 hypothetical protein -
AFK66_RS20645 11859..12596 + 738 WP_029039441.1 DUF4145 domain-containing protein -
AFK66_RS20650 13074..13799 - 726 WP_042391453.1 conjugal transfer protein TraX -
AFK66_RS22570 14303..14902 - 600 WP_105166309.1 hypothetical protein -
AFK66_RS20660 15005..15502 - 498 WP_007778984.1 hypothetical protein -
AFK66_RS20665 15551..15967 - 417 WP_007778986.1 type II toxin-antitoxin system VapC family toxin Toxin
AFK66_RS20670 15964..16194 - 231 WP_007778988.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AFK66_RS20675 16374..16586 + 213 WP_012815904.1 hypothetical protein -
AFK66_RS20680 16672..17232 - 561 WP_012815905.1 hypothetical protein -
AFK66_RS20685 17299..19494 - 2196 WP_007778991.1 phage T7 exclusion protein -
AFK66_RS20690 19495..20580 - 1086 WP_007889785.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..52622 52622


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14946.37 Da        Isoelectric Point: 7.8858

>T59630 WP_007778986.1 NZ_CP013942:c15967-15551 [Cronobacter malonaticus LMG 23826]
VKKTYMLDTNICSFIMREQPEAVLKQLEQTVLRGHRIVVSAITYAEMRFGAIGKKASPRHAVLVEAFCARLDAVLAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLMLEDWTK

Download         Length: 417 bp

>T59630 NZ_CP013942:c15967-15551 [Cronobacter malonaticus LMG 23826]
GTGAAAAAGACCTATATGCTCGATACCAATATCTGTTCATTCATCATGCGTGAACAGCCCGAAGCGGTACTGAAGCAGCT
GGAGCAGACGGTACTGCGGGGACACCGGATTGTCGTCTCAGCCATTACCTATGCCGAAATGCGGTTCGGTGCCATCGGCA
AAAAAGCCTCCCCGCGTCACGCGGTGCTGGTTGAGGCATTCTGTGCGCGGCTTGATGCCGTTCTGGCGTGGGACCGGGCC
GCGGTGGACGCGACCACAGAAATCAAGGCGGCGCTGACCGCGGCCGGCACCCCGATAGGTCCGAATGATACGGCGATTGC
CGGGCATGCCATTGCAGCCGGCGCCGTACTGGTTACCAATAATGTGCGTGAATTTGAACGTGTTCCGGGACTGATGCTGG
AAGACTGGACGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8683.80 Da        Isoelectric Point: 4.4432

>AT59630 WP_007778988.1 NZ_CP013942:c16194-15964 [Cronobacter malonaticus LMG 23826]
MRTVSVFKNGQNRAIRLPRDMDFEGVSELEIIREGDSIILRPVRPTWGSFAQLDKADPDFMAERADVVSDEGRFDL

Download         Length: 231 bp

>AT59630 NZ_CP013942:c16194-15964 [Cronobacter malonaticus LMG 23826]
ATGCGTACTGTGTCTGTATTTAAAAATGGCCAGAACCGGGCTATCCGGCTGCCGCGGGATATGGATTTTGAGGGCGTCAG
CGAGCTGGAAATCATCCGGGAAGGCGACAGTATCATCCTGCGTCCCGTCCGCCCGACGTGGGGCTCCTTCGCGCAGCTCG
ACAAAGCAGATCCGGACTTTATGGCGGAGCGCGCCGACGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7URM1

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7RF87

References