Detailed information of TA system TA059629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 6826..8429
• Replicon: plasmid pCMA1
• Accession: NZ_CP013941
• Organism: Cronobacter malonaticus LMG 23826

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V5U5S2
• Locus tag: AFK66_RS20095
• Protein ID: WP_023897618.1
• Coordinates: 6826..8145 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: AFK66_RS20100
• Protein ID: WP_007781034.1
• Coordinates: 8148..8429 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK66_RS20085	2207..4738	-	2532	WP_023897681.1	malto-oligosyltrehalose synthase	-
AFK66_RS20090	4735..6519	-	1785	WP_032968604.1	malto-oligosyltrehalose trehalohydrolase	-
AFK66_RS20095	6826..8145	-	1320	WP_023897618.1	type II toxin-antitoxin system HipA family toxin	Toxin
AFK66_RS20100	8148..8429	-	282	WP_007781034.1	helix-turn-helix domain-containing protein	Antitoxin
AFK66_RS20105	8562..9446	-	885	WP_023897619.1	siderophore-interacting protein	-
AFK66_RS20110	9443..10654	-	1212	WP_007780883.1	MFS transporter	-
AFK66_RS20115	10790..12538	+	1749	WP_032983474.1	aerobactin synthase IucA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iucA / iucB / iucC / iutA / mgtB	1..126500	126500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (153-405)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49008.97 Da
• Isoelectric Point: 7.2132

>T59629 WP_023897618.1 NZ_CP013941:c8145-6826 [Cronobacter malonaticus LMG 23826]
MRRTSRLALWMNGLPVGYWDKHNGEDRLTYLPEWIADPQGRPLSLSLPFTPGNQSYRGARVRDYFDNLLPDSENIRRRLA
MRYRAQSLEPFDLLAELGRDCVGAIQLLPAGEAPEGLFTIRARPLSTVDIAAMLRHTAEAFPGQHGGEEELRLSIAGAQE
KTALLWHEGQWCLPEGNTPTTHIFKLPLGLVGNMQADMRTSIENEWLCAQLLEAWDIPVARTQMAQFEEQKVLVVERFDR
RLSADGQWWLRLPQEDMCQALGVSPLRKYQADGGPGIGDIMTVLSRSENAQADRANFFRAQIIFWILAATDGHAKNFSIA
HLPGNRYHLTPLYDVLSAWPVIGPGNNQIAWQKCKLAMAVRSGSNYYHLSQIRRRHWLRQGELAGLSAAQVNDMIDALTA
SAPAAIARVAGTLPVDFPASLADVIFNGIRQQCARLAAA

Nucleotide

• Length: 1320 bp

>T59629 NZ_CP013941:c8145-6826 [Cronobacter malonaticus LMG 23826]
ATGCGCCGCACGTCGCGTTTAGCGCTCTGGATGAACGGCCTCCCGGTCGGCTACTGGGATAAACACAATGGCGAAGACCG
GCTGACCTATCTGCCGGAGTGGATTGCCGATCCGCAAGGGCGGCCGCTGTCGCTGTCGCTGCCGTTCACGCCCGGTAATC
AGTCATACCGCGGCGCGCGGGTGCGCGATTACTTCGACAACCTGCTGCCGGACAGCGAAAACATCCGGCGGCGGCTGGCG
ATGCGCTACCGGGCGCAGAGCCTGGAGCCGTTCGATCTGCTGGCGGAGCTGGGACGCGACTGCGTCGGCGCTATTCAGCT
CCTGCCCGCCGGTGAAGCGCCGGAAGGGCTGTTTACCATCCGCGCCCGTCCACTCTCCACAGTGGATATTGCCGCGATGC
TGCGCCACACCGCAGAGGCGTTCCCCGGCCAGCATGGCGGCGAAGAGGAGCTGCGGCTCTCCATTGCGGGCGCGCAGGAG
AAAACCGCGCTGCTGTGGCACGAAGGCCAGTGGTGCCTGCCGGAGGGCAACACGCCCACCACGCATATTTTCAAACTGCC
GCTGGGGCTGGTGGGCAATATGCAGGCGGACATGCGCACCTCGATTGAAAACGAATGGCTCTGCGCGCAGCTGCTGGAAG
CCTGGGATATTCCGGTCGCGCGCACGCAGATGGCGCAGTTCGAAGAGCAAAAAGTGCTGGTTGTCGAGCGCTTCGACCGG
CGTCTCTCGGCCGATGGCCAGTGGTGGCTGCGTCTGCCGCAGGAGGATATGTGCCAGGCGCTGGGCGTCTCGCCGCTGCG
TAAATATCAGGCCGACGGCGGGCCGGGCATCGGCGATATCATGACGGTTCTGAGCCGTTCAGAAAACGCGCAGGCGGATC
GCGCGAATTTCTTCCGCGCGCAGATTATCTTCTGGATCCTCGCCGCGACCGACGGCCACGCCAAGAATTTCAGCATCGCG
CATCTGCCCGGCAACCGCTACCATCTGACGCCGCTTTACGACGTGCTTTCCGCCTGGCCGGTCATCGGGCCGGGTAATAA
CCAGATTGCGTGGCAGAAGTGCAAGCTGGCGATGGCGGTGCGCAGCGGCAGTAACTATTACCACCTGAGCCAGATCCGCC
GCCGTCACTGGCTGCGCCAGGGCGAGCTTGCCGGGCTGTCGGCGGCGCAGGTCAACGACATGATTGATGCGCTGACAGCC
AGCGCGCCCGCGGCGATCGCGCGCGTGGCCGGCACGCTGCCGGTGGATTTCCCCGCTTCTCTGGCAGACGTTATTTTTAA
CGGCATACGCCAGCAGTGCGCGCGCCTCGCGGCCGCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 8.0115

>AT59629 WP_007781034.1 NZ_CP013941:c8429-8148 [Cronobacter malonaticus LMG 23826]
MNTHYPLKTLSQLRPLLVGFRKNQGLTQKDVSARLGVTQQTYARLEANPETASIERLFRVFNVLGIEMTLCETQAAEADA
PARDDSPARREAW

Nucleotide

• Length: 282 bp

>AT59629 NZ_CP013941:c8429-8148 [Cronobacter malonaticus LMG 23826]
ATGAATACCCATTATCCACTGAAAACGCTCAGCCAGCTGCGCCCGCTGCTGGTGGGCTTTCGTAAAAATCAGGGGCTGAC
CCAGAAAGACGTCTCCGCGCGGCTTGGCGTCACGCAGCAAACCTATGCGCGGCTGGAGGCGAACCCCGAGACGGCCAGCA
TTGAGCGCCTGTTCCGCGTCTTTAACGTGCTGGGCATTGAGATGACGCTGTGCGAAACGCAGGCGGCAGAAGCTGACGCG
CCCGCGCGTGACGATTCGCCTGCCCGTCGCGAGGCGTGGTAA

Structures

Toxin

• AlphaFold DB: V5U5S2