Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 3216129..3216808 Replicon chromosome
Accession NZ_CP013940
Organism Cronobacter malonaticus LMG 23826

Toxin (Protein)


Gene name ykfI Uniprot ID A0A8B3US07
Locus tag AFK66_RS15145 Protein ID WP_007777710.1
Coordinates 3216467..3216808 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A8B3USS8
Locus tag AFK66_RS15140 Protein ID WP_007777712.1
Coordinates 3216129..3216446 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK66_RS15110 3211822..3212802 - 981 WP_007777755.1 IS5 family transposase -
AFK66_RS15125 3214181..3214555 + 375 Protein_3025 50S ribosome-binding GTPase -
AFK66_RS21645 3214587..3214826 + 240 WP_029039036.1 DUF905 domain-containing protein -
AFK66_RS15130 3214928..3215386 + 459 WP_023899364.1 antirestriction protein -
AFK66_RS15135 3215402..3215878 + 477 WP_029039034.1 RadC family protein -
AFK66_RS21650 3215887..3216108 + 222 WP_007777714.1 DUF987 domain-containing protein -
AFK66_RS15140 3216129..3216446 + 318 WP_007777712.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AFK66_RS15145 3216467..3216808 + 342 WP_007777710.1 TA system toxin CbtA family protein Toxin
AFK66_RS15150 3217540..3218793 - 1254 WP_007777708.1 hypothetical protein -
AFK66_RS15155 3219160..3219624 - 465 WP_032968632.1 helix-turn-helix domain-containing protein -
AFK66_RS15160 3219864..3220361 + 498 WP_032967861.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3168977..3229372 60395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12934.88 Da        Isoelectric Point: 8.0308

>T59627 WP_007777710.1 NZ_CP013940:3216467-3216808 [Cronobacter malonaticus LMG 23826]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLQAVDILRARQATGLLQQSRSNVVR

Download         Length: 342 bp

>T59627 NZ_CP013940:3216467-3216808 [Cronobacter malonaticus LMG 23826]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCGCCTTATCTTCAGGCTGTCGATATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCAGCAAAG
CCGTAGCAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12005.67 Da        Isoelectric Point: 7.3723

>AT59627 WP_007777712.1 NZ_CP013940:3216129-3216446 [Cronobacter malonaticus LMG 23826]
MTHITWGLQRNITPRLGARLVQEGNRLHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVIMYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT59627 NZ_CP013940:3216129-3216446 [Cronobacter malonaticus LMG 23826]
ATGACTCACATCACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCATGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGTTACGTGTACATCGCCATTTATCCCACCCAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3US07

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3USS8

References