Detailed information of TA system TA059626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2983596..2984215
• Replicon: chromosome
• Accession: NZ_CP013940
• Organism: Cronobacter malonaticus LMG 23826

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V5U1U2
• Locus tag: AFK66_RS14055
• Protein ID: WP_007781479.1
• Coordinates: 2983997..2984215 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V5U2H5
• Locus tag: AFK66_RS14050
• Protein ID: WP_007781481.1
• Coordinates: 2983596..2983970 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK66_RS14040	2978756..2979961	+	1206	WP_007781488.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
AFK66_RS14045	2979983..2983132	+	3150	WP_007781486.1	multidrug efflux RND transporter permease subunit	-
AFK66_RS14050	2983596..2983970	+	375	WP_007781481.1	Hha toxicity modulator TomB	Antitoxin
AFK66_RS14055	2983997..2984215	+	219	WP_007781479.1	hemolysin expression modulator Hha	Toxin
AFK66_RS14060	2984420..2984971	+	552	WP_007781478.1	maltose O-acetyltransferase	-
AFK66_RS14065	2985092..2985601	+	510	WP_007791512.1	YlaC family protein	-
AFK66_RS21555	2985633..2985773	-	141	WP_004386911.1	type B 50S ribosomal protein L36	-
AFK66_RS14070	2985777..2986037	-	261	WP_004386912.1	type B 50S ribosomal protein L31	-
AFK66_RS14075	2986177..2988078	-	1902	WP_007781473.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8614.02 Da
• Isoelectric Point: 9.4828

>T59626 WP_007781479.1 NZ_CP013940:2983997-2984215 [Cronobacter malonaticus LMG 23826]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T59626 NZ_CP013940:2983997-2984215 [Cronobacter malonaticus LMG 23826]
ATGACGACCAAACCATTAACTAAAACCGATTATTTAATGCGACTGAGACGCTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAATGAACTGGCGGTATTTTATTCAGCAGCGGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAATCCCGACCTCCGTGTGGAAATTCGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14357.08 Da
• Isoelectric Point: 4.7168

>AT59626 WP_007781481.1 NZ_CP013940:2983596-2983970 [Cronobacter malonaticus LMG 23826]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFGLNYKIKHEDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFINASKENPVSLSF

Nucleotide

• Length: 375 bp

>AT59626 NZ_CP013940:2983596-2983970 [Cronobacter malonaticus LMG 23826]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTCTGTGAGAGCCTGTATCATGATTGCCTCGC
CACGCTAGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCCGCCGTTAACCTGCAACTGAACGACCTGATTGAGC
ATATTGCCAGCTTCGGTCTTAATTATAAAATTAAGCATGAAGATGACGCCGCTCTCATTGAACAATTAGATGAATATCTG
GACGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTCATTAACGCCAGTAAAGAAAATCCTGTGAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: V5U1U2

Antitoxin

• AlphaFold DB: V5U2H5