Detailed information of TA system TA059614

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 624837..625500
• Replicon: chromosome
• Accession: NZ_CP013940
• Organism: Cronobacter malonaticus LMG 23826

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: V5TX49
• Locus tag: AFK66_RS02965
• Protein ID: WP_007777930.1
• Coordinates: 625084..625500 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V5TVG3
• Locus tag: AFK66_RS02960
• Protein ID: WP_007777934.1
• Coordinates: 624837..625103 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK66_RS02935	619865..621298	-	1434	WP_007777946.1	6-phospho-beta-glucosidase	-
AFK66_RS02940	621448..622176	-	729	WP_032969013.1	MurR/RpiR family transcriptional regulator	-
AFK66_RS02945	622352..622666	+	315	WP_007777940.1	N(4)-acetylcytidine aminohydrolase	-
AFK66_RS02950	622809..623462	+	654	WP_007777939.1	hemolysin III family protein	-
AFK66_RS02955	623570..624559	-	990	WP_007777936.1	tRNA-modifying protein YgfZ	-
AFK66_RS02960	624837..625103	+	267	WP_007777934.1	FAD assembly factor SdhE	Antitoxin
AFK66_RS02965	625084..625500	+	417	WP_007777930.1	protein YgfX	Toxin
AFK66_RS02970	625514..626035	-	522	WP_007777929.1	flavodoxin FldB	-
AFK66_RS02975	626137..627033	+	897	WP_023898044.1	site-specific tyrosine recombinase XerD	-
AFK66_RS02980	627056..627775	+	720	WP_007777924.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
AFK66_RS02985	627779..629512	+	1734	WP_007777920.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16309.25 Da
• Isoelectric Point: 11.0765

>T59614 WP_007777930.1 NZ_CP013940:625084-625500 [Cronobacter malonaticus LMG 23826]
VVLWHSDLRVSWRSQWVSLLLHGAVAVIILLLPWPLRYLPVWMLLLSLVVFDCVRSQRRINAFQGEVSLTADYQLRWQGV
DWQICSTPWMLRSGMMLRLRHPKTTRRHHVWLAADSMTAAEWRDLCRLLLQQPVGDNR

Nucleotide

• Length: 417 bp

>T59614 NZ_CP013940:625084-625500 [Cronobacter malonaticus LMG 23826]
GTGGTCCTGTGGCACTCTGATCTTCGCGTTTCATGGCGCTCGCAGTGGGTATCGCTGCTGCTGCATGGCGCTGTGGCTGT
TATTATTTTGCTGCTTCCGTGGCCGCTGCGGTATCTGCCCGTCTGGATGCTGTTGCTGTCTCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGTATCAATGCGTTTCAGGGTGAAGTCAGCCTGACGGCTGATTATCAGCTACGATGGCAGGGCGTG
GACTGGCAGATTTGCTCCACGCCCTGGATGTTGCGTAGCGGAATGATGCTGCGGCTGCGTCATCCGAAAACGACGCGCCG
CCATCACGTCTGGCTGGCGGCGGATAGTATGACGGCAGCGGAGTGGCGCGATTTGTGTCGTCTTTTGTTGCAACAGCCCG
TGGGCGACAACCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10441.92 Da
• Isoelectric Point: 4.8310

>AT59614 WP_007777934.1 NZ_CP013940:624837-625103 [Cronobacter malonaticus LMG 23826]
MDINNKARIHWACRRGMRELDISIMPFFEYDYDSLSDEDKRVFVRLLECDDPDLFNWLMNHGKPADTELARMVELIQQRN
QARGPVAL

Nucleotide

• Length: 267 bp

>AT59614 NZ_CP013940:624837-625103 [Cronobacter malonaticus LMG 23826]
ATGGATATTAATAATAAAGCCCGTATTCACTGGGCCTGTCGCAGAGGTATGCGCGAGCTGGATATTTCCATCATGCCTTT
CTTTGAATACGACTACGACTCCCTGAGCGATGAAGACAAACGCGTTTTTGTTCGCCTGCTGGAGTGTGACGATCCTGATT
TATTTAACTGGCTGATGAATCATGGCAAACCTGCCGATACGGAGCTGGCGCGAATGGTGGAATTAATCCAGCAACGGAAT
CAGGCACGTGGTCCTGTGGCACTCTGA

Structures

Toxin

• AlphaFold DB: V5TX49

Antitoxin

• AlphaFold DB: V5TVG3