Detailed information of TA system TA059589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 5709529..5710136
• Replicon: chromosome
• Accession: NZ_CP013923
• Organism: Achromobacter denitrificans strain USDA-ARS-USMARC-56712

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A848NDJ8
• Locus tag: APT56_RS25480
• Protein ID: WP_059272679.1
• Coordinates: 5709828..5710136 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: APT56_RS25475
• Protein ID: WP_059272678.1
• Coordinates: 5709529..5709831 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT56_RS28305	5704602..5705681	+	1080	WP_082707231.1	DUF4297 domain-containing protein	-
APT56_RS28680	5705756..5707465	+	1710	WP_156430361.1	hypothetical protein	-
APT56_RS28310	5707587..5709209	+	1623	WP_167352802.1	RNA-directed DNA polymerase	-
APT56_RS25475	5709529..5709831	-	303	WP_059272678.1	putative addiction module antidote protein	Antitoxin
APT56_RS25480	5709828..5710136	-	309	WP_059272679.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APT56_RS25485	5710283..5710750	+	468	WP_059272680.1	hypothetical protein	-
APT56_RS25490	5710782..5711105	-	324	WP_059272681.1	YXWGXW repeat-containing protein	-
APT56_RS25495	5711399..5712415	+	1017	WP_059272682.1	amino acid ABC transporter substrate-binding protein	-
APT56_RS25500	5712613..5712894	+	282	WP_006385706.1	peptidyl-prolyl cis-trans isomerase	-
APT56_RS25505	5713124..5713864	-	741	WP_059273114.1	sugar ABC transporter ATP-binding protein	-
APT56_RS25510	5713940..5714899	-	960	WP_054414631.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11619.62 Da
• Isoelectric Point: 10.9528

>T59589 WP_059272679.1 NZ_CP013923:c5710136-5709828 [Achromobacter denitrificans]
MLRFRETDVFSRWILGLRDMRAKALILRRLARAMAGHFGDIAPVGDGISEMRIDAGAGYRVYYRRQGQDLYLLLCGGDKK
SQARDIATAKRLWAELKGKAFK

Nucleotide

• Length: 309 bp

>T59589 NZ_CP013923:c5710136-5709828 [Achromobacter denitrificans]
ATGTTGAGGTTCCGCGAAACCGACGTTTTTTCAAGATGGATCTTGGGGTTGCGGGACATGCGGGCCAAGGCGCTGATCTT
GCGTCGGCTGGCCCGTGCAATGGCCGGGCATTTCGGCGATATAGCGCCTGTTGGTGACGGCATCAGTGAAATGCGGATCG
ACGCGGGCGCTGGGTATCGGGTCTATTACCGCCGCCAGGGGCAAGACCTGTACTTGCTGCTGTGCGGTGGCGACAAGAAG
TCCCAGGCGCGCGACATTGCGACTGCGAAGCGGCTATGGGCCGAACTCAAAGGGAAGGCATTCAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10559.92 Da
• Isoelectric Point: 4.7066

>AT59589 WP_059272678.1 NZ_CP013923:c5709831-5709529 [Achromobacter denitrificans]
MTQLRDFEPANYLADDETIAHYLADAAADPDPDAFLQALGDVARARGIGAIAKETGLARTNLYRVLAPGANPSYLTLRRV
MDALGVSLTAVAKGKDTAQA

Nucleotide

• Length: 303 bp

>AT59589 NZ_CP013923:c5709831-5709529 [Achromobacter denitrificans]
ATGACCCAGTTGCGTGATTTCGAGCCGGCGAATTATCTGGCCGATGACGAGACGATTGCGCATTACCTGGCGGATGCGGC
GGCCGATCCCGATCCGGACGCTTTTCTCCAGGCGCTTGGCGATGTTGCCCGCGCGCGCGGTATCGGGGCCATTGCGAAGG
AGACCGGCCTGGCGCGCACGAACCTGTACCGCGTGCTCGCGCCGGGCGCCAACCCAAGCTACCTGACGCTGCGCCGGGTC
ATGGATGCATTGGGCGTATCGCTCACGGCCGTCGCCAAGGGCAAGGACACCGCGCAGGCATAA

Structures

Toxin

• AlphaFold DB: A0A848NDJ8