Detailed information of TA system TA059587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1166658..1167231
• Replicon: chromosome
• Accession: NZ_CP013923
• Organism: Achromobacter denitrificans strain USDA-ARS-USMARC-56712

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5K4XXH8
• Locus tag: APT56_RS05190
• Protein ID: WP_049071622.1
• Coordinates: 1166854..1167231 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: APT56_RS27955
• Protein ID: WP_081330505.1
• Coordinates: 1166658..1166867 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT56_RS05170	1162043..1162852	-	810	WP_059269404.1	helix-turn-helix transcriptional regulator	-
APT56_RS05175	1162952..1163878	+	927	WP_080977321.1	DMT family transporter	-
APT56_RS05180	1163892..1164710	-	819	WP_059269405.1	AAC(3) family N-acetyltransferase	-
APT56_RS05185	1164861..1166534	+	1674	WP_006388972.1	energy-dependent translational throttle protein EttA	-
APT56_RS27955	1166658..1166867	+	210	WP_081330505.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
APT56_RS05190	1166854..1167231	+	378	WP_049071622.1	type II toxin-antitoxin system VapC family toxin	Toxin
APT56_RS05195	1167299..1167832	+	534	WP_059269406.1	isochorismatase family protein	-
APT56_RS05200	1168023..1168247	+	225	WP_049071625.1	glycine zipper 2TM domain-containing protein	-
APT56_RS05205	1168379..1168636	-	258	WP_006388977.1	cell division topological specificity factor MinE	-
APT56_RS05210	1168640..1169455	-	816	WP_049077490.1	septum site-determining protein MinD	-
APT56_RS05215	1169644..1170573	-	930	WP_049071626.1	septum site-determining protein MinC	-
APT56_RS05220	1170664..1171395	-	732	WP_059269407.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13910.18 Da
• Isoelectric Point: 6.4885

>T59587 WP_049071622.1 NZ_CP013923:1166854-1167231 [Achromobacter denitrificans]
MILVDTSIWIDHLSDGEPYLVRLLEEESVLMHPFIAGELALGSLGRRDVVLGAMSLLPQVVRASHDEVMHFLQSEKLFGK
GIGYVDLHLLASTRLTPGAALWTRDKRLRALANTLSLAVDPPRYH

Nucleotide

• Length: 378 bp

>T59587 NZ_CP013923:1166854-1167231 [Achromobacter denitrificans]
ATGATCCTGGTTGACACTTCCATCTGGATAGACCATTTATCCGACGGCGAGCCATATCTGGTGCGCTTGCTGGAGGAAGA
AAGTGTATTGATGCACCCGTTCATCGCGGGTGAATTGGCGCTGGGTTCGTTGGGCCGACGTGATGTCGTATTGGGCGCGA
TGTCGCTATTGCCTCAAGTCGTGCGTGCGAGTCATGACGAAGTCATGCACTTTCTTCAAAGCGAGAAATTGTTTGGAAAG
GGCATCGGCTATGTGGATTTACACTTGTTGGCGTCCACGCGACTGACACCTGGCGCTGCGCTATGGACCCGGGACAAACG
GCTGCGGGCACTCGCCAACACCTTGAGCCTGGCGGTTGATCCGCCGAGATATCATTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7491.59 Da
• Isoelectric Point: 9.9435

>AT59587 WP_081330505.1 NZ_CP013923:1166658-1166867 [Achromobacter denitrificans]
VRTTVTLDDDLLELARAYTGVQETSALVTRALTQLVQKEAAKRLAKLGGSSPKLKPIPRRQTETCNDPG

Nucleotide

• Length: 210 bp

>AT59587 NZ_CP013923:1166658-1166867 [Achromobacter denitrificans]
GTGCGTACGACTGTGACGCTCGATGACGACCTGCTCGAATTGGCCCGTGCCTATACCGGCGTCCAGGAGACATCGGCGCT
CGTAACGCGAGCGTTGACCCAGCTCGTGCAGAAAGAGGCCGCGAAGCGGTTGGCCAAGCTTGGTGGTAGTTCGCCCAAGT
TGAAGCCTATCCCGCGGCGGCAGACGGAAACCTGCAATGATCCTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A5K4XXH8