Detailed information of TA system TA059585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1595913..1596442
• Replicon: chromosome
• Accession: NZ_CP013922
• Organism: Staphylococcus xylosus strain S170

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AWC37_RS07870
• Protein ID: WP_029377956.1
• Coordinates: 1595913..1596275 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D4YKY9
• Locus tag: AWC37_RS07875
• Protein ID: WP_017722121.1
• Coordinates: 1596272..1596442 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC37_RS07850	1592928..1593698	-	771	WP_038678880.1	RNA polymerase sigma factor SigB	-
AWC37_RS07855	1593673..1594155	-	483	WP_029377953.1	anti-sigma B factor RsbW	-
AWC37_RS07860	1594157..1594483	-	327	WP_029377954.1	anti-sigma factor antagonist	-
AWC37_RS07865	1594563..1595564	-	1002	WP_029377955.1	PP2C family protein-serine/threonine phosphatase	-
AWC37_RS07870	1595913..1596275	-	363	WP_029377956.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWC37_RS07875	1596272..1596442	-	171	WP_017722121.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AWC37_RS07880	1596530..1597678	-	1149	WP_029377957.1	alanine racemase	-
AWC37_RS07885	1597792..1598145	-	354	WP_029377958.1	holo-ACP synthase	-
AWC37_RS07890	1598207..1598698	-	492	WP_029377959.1	PH domain-containing protein	-
AWC37_RS07895	1598685..1600193	-	1509	WP_069796645.1	PH domain-containing protein	-
AWC37_RS07900	1600186..1600665	-	480	WP_029377961.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13539.75 Da
• Isoelectric Point: 10.3173

>T59585 WP_029377956.1 NZ_CP013922:c1596275-1595913 [Staphylococcus xylosus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEKKMKEVNTAIDISLGLHNIRNHKS

Nucleotide

• Length: 363 bp

>T59585 NZ_CP013922:c1596275-1595913 [Staphylococcus xylosus]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTACAAGGGTCTGAACAAGGGGGAATTAGACCCGTCGT
TATTATACAAAACGATACTGGTAACAAATATAGTCCTACAGTAATTGTAGCTGCAATTACTGGTAGGATTAATAAAGCTA
AAATACCTACACATGTAGAGATAGAAAAAAGTAAATATAAACTTGATAAAGATTCTGTTATATTGTTAGAACAAATCAGA
ACTTTAGATAAAAATAGGCTAAAGGAAAAATTAACATATCTTTCTGAGAAAAAAATGAAAGAAGTTAATACTGCTATTGA
TATAAGTTTAGGATTACATAATATTAGGAATCATAAATCCTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT59585 WP_017722121.1 NZ_CP013922:c1596442-1596272 [Staphylococcus xylosus]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT59585 NZ_CP013922:c1596442-1596272 [Staphylococcus xylosus]
ATGGCAAGTTTTAATCAAAGTAGAAATCATAGTATTGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGACTTAAA
TCTCTCCCTTGCAGCAGAAGCCTTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACATTAGCTCTAAGAATA
ATAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1D4YKY9