Detailed information of TA system TA059571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1644593..1645125
• Replicon: chromosome
• Accession: NZ_CP013911
• Organism: Staphylococcus haemolyticus strain S167

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q4L7V3
• Locus tag: AV904_RS07945
• Protein ID: WP_011275272.1
• Coordinates: 1644593..1644958 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AV904_RS07950
• Protein ID: WP_053038754.1
• Coordinates: 1644955..1645125 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AV904_RS07925	1641633..1642403	-	771	WP_011275276.1	RNA polymerase sigma factor SigB	-
AV904_RS07930	1642378..1642857	-	480	WP_011275275.1	anti-sigma B factor RsbW	-
AV904_RS07935	1642859..1643185	-	327	WP_011275274.1	anti-sigma factor antagonist	-
AV904_RS07940	1643284..1644285	-	1002	WP_037559762.1	PP2C family protein-serine/threonine phosphatase	-
AV904_RS07945	1644593..1644958	-	366	WP_011275272.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AV904_RS07950	1644955..1645125	-	171	WP_053038754.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AV904_RS07955	1645211..1646359	-	1149	WP_016931365.1	alanine racemase	-
AV904_RS07960	1646455..1646814	-	360	WP_037559766.1	holo-ACP synthase	-
AV904_RS07965	1646824..1647351	-	528	WP_016931364.1	PH domain-containing protein	-
AV904_RS07970	1647344..1648867	-	1524	WP_016931363.1	PH domain-containing protein	-
AV904_RS07975	1648860..1649339	-	480	WP_011275266.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	SCCmec	-	groEL	1262607..1692661	430054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13672.75 Da
• Isoelectric Point: 8.4121

>T59571 WP_011275272.1 NZ_CP013911:c1644958-1644593 [Staphylococcus haemolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYRLDKDSVILLEQIR
TLDKKRLKEKLTYLSDEKMQEVDEALDISLGLHDEVKTQNT

Nucleotide

• Length: 366 bp

>T59571 NZ_CP013911:c1644958-1644593 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGTAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTTATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTGGATGAAGCGTTAGA
CATTAGTCTAGGATTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6420.10 Da
• Isoelectric Point: 4.2120

>AT59571 WP_053038754.1 NZ_CP013911:c1645125-1644955 [Staphylococcus haemolyticus]
MLTFNQNRSHSLEQSLKEGYAQMAELNLSLATEAFHLECEACDCNETYLISNYKSE

Nucleotide

• Length: 171 bp

>AT59571 NZ_CP013911:c1645125-1644955 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATGCACAAATGGCCGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCATCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACCTTATTTCTAATTATA
AAAGTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2A1KA75