Detailed information of TA system TA059473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2292116..2292768
• Replicon: chromosome
• Accession: NZ_CP013862
• Organism: Lentibacillus amyloliquefaciens strain LAM0015

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0U4F0V9
• Locus tag: AOX59_RS11450
• Protein ID: WP_068445650.1
• Coordinates: 2292116..2292475 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AOX59_RS11455
• Protein ID: WP_177183463.1
• Coordinates: 2292481..2292768 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX59_RS11415	2287190..2287981	-	792	WP_068445634.1	RNA polymerase sigma factor SigB	-
AOX59_RS11420	2287953..2288429	-	477	WP_068445638.1	anti-sigma B factor RsbW	-
AOX59_RS11425	2288429..2288761	-	333	WP_068445642.1	STAS domain-containing protein	-
AOX59_RS11430	2288998..2290017	-	1020	WP_068445643.1	PP2C family protein-serine/threonine phosphatase	-
AOX59_RS11435	2290100..2290501	-	402	WP_068445645.1	anti-sigma regulatory factor	-
AOX59_RS11440	2290504..2290869	-	366	WP_179946404.1	STAS domain-containing protein	-
AOX59_RS11445	2290866..2291735	-	870	WP_068445648.1	RsbT co-antagonist protein RsbRA	-
AOX59_RS11450	2292116..2292475	-	360	WP_068445650.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AOX59_RS11455	2292481..2292768	-	288	WP_177183463.1	antitoxin	Antitoxin
AOX59_RS11460	2293481..2294602	-	1122	WP_068445654.1	alanine racemase	-
AOX59_RS11465	2294708..2295724	-	1017	WP_068445656.1	outer membrane lipoprotein carrier protein LolA	-
AOX59_RS11470	2295810..2297327	-	1518	WP_068445658.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
AOX59_RS11475	2297392..2297754	-	363	WP_068445660.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13463.67 Da
• Isoelectric Point: 8.4843

>T59473 WP_068445650.1 NZ_CP013862:c2292475-2292116 [Lentibacillus amyloliquefaciens]
MIVQRGEVYFADLSPVVGSEQGGIRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDHQMMKKIDNALEISLGLKEMYDQ

Nucleotide

• Length: 360 bp

>T59473 NZ_CP013862:c2292475-2292116 [Lentibacillus amyloliquefaciens]
TTGATCGTTCAAAGAGGCGAAGTTTATTTCGCCGACTTATCCCCCGTTGTGGGATCAGAACAGGGAGGCATACGGCCGGT
CTTGATCCTGCAGAATGATATCGGAAACCGCTTCAGCCCAACGGTTATTGTTGCTGCTATAACTGCTCAGATTCAGAAGG
CCAAACTCCCGACACATGTGGAGATCGATGCGAAACGGTATGGATTCGACCGTAACTCGGTTATATTGCTGGAACAAATA
AGAACGTTGGATAAGCAGCGGTTGACCGATAAAATAACAAAACTTGATCATCAGATGATGAAAAAGATTGATAACGCTTT
AGAGATCAGTCTTGGACTGAAAGAAATGTATGATCAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10999.27 Da
• Isoelectric Point: 4.4335

>AT59473 WP_177183463.1 NZ_CP013862:c2292768-2292481 [Lentibacillus amyloliquefaciens]
MSESSQEISVRLPKNLLNEVDGLMKYENSNLSDFICQATRNYLEHKKEEHVQQFRESMQQGYMEMANINLTIASEAFQAE
EEAEKNNLQRTVIGV

Nucleotide

• Length: 288 bp

>AT59473 NZ_CP013862:c2292768-2292481 [Lentibacillus amyloliquefaciens]
TTGTCAGAAAGCTCACAAGAAATTTCAGTACGCTTACCAAAGAACCTGTTAAATGAGGTGGATGGACTAATGAAATATGA
AAACAGTAATCTGAGTGATTTCATATGTCAGGCAACGCGAAATTATCTGGAACACAAAAAAGAAGAGCATGTCCAACAGT
TTCGTGAATCAATGCAGCAAGGGTACATGGAAATGGCTAATATTAATCTCACCATCGCTTCAGAAGCTTTTCAGGCCGAG
GAAGAAGCTGAGAAAAACAACTTACAGCGCACAGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0U4F0V9