Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2278227..2279058 Replicon chromosome
Accession NZ_CP013835
Organism Escherichia coli strain JJ2434

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag AVR68_RS12890 Protein ID WP_000854815.1
Coordinates 2278684..2279058 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag AVR68_RS12885 Protein ID WP_001280918.1
Coordinates 2278227..2278595 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR68_RS12840 2273316..2274062 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
AVR68_RS12845 2274145..2274495 + 351 Protein_2253 hypothetical protein -
AVR68_RS12850 2274511..2274921 + 411 WP_000846703.1 hypothetical protein -
AVR68_RS12855 2275142..2275960 + 819 WP_001542275.1 DUF945 domain-containing protein -
AVR68_RS12860 2275960..2276205 + 246 WP_001164966.1 hypothetical protein -
AVR68_RS12865 2276299..2276772 + 474 WP_001542276.1 antirestriction protein -
AVR68_RS12870 2276788..2277264 + 477 WP_001186200.1 RadC family protein -
AVR68_RS12875 2277327..2277548 + 222 WP_000692345.1 DUF987 domain-containing protein -
AVR68_RS12880 2277567..2278211 + 645 WP_000086752.1 hypothetical protein -
AVR68_RS12885 2278227..2278595 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AVR68_RS12890 2278684..2279058 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
AVR68_RS12895 2279055..2279249 + 195 WP_000988600.1 hypothetical protein -
AVR68_RS29900 2279262..2279375 + 114 WP_001161660.1 DUF957 domain-containing protein -
AVR68_RS12905 2279664..2279810 - 147 Protein_2265 transposase domain-containing protein -
AVR68_RS12910 2279864..2280046 + 183 WP_001296208.1 hypothetical protein -
AVR68_RS12915 2280147..2280476 - 330 WP_000450409.1 DUF496 family protein -
AVR68_RS12920 2280648..2281706 - 1059 WP_001200889.1 FUSC family protein -
AVR68_RS12925 2281904..2282377 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
AVR68_RS12930 2282496..2283662 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T59454 WP_000854815.1 NZ_CP013835:2278684-2279058 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T59454 NZ_CP013835:2278684-2279058 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT59454 WP_001280918.1 NZ_CP013835:2278227-2278595 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT59454 NZ_CP013835:2278227-2278595 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References