59427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 112056..112615 Replicon plasmid pJJ2434_1
Accession NZ_CP013833
Organism Escherichia coli strain JJ2434

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag AVR68_RS00650 Protein ID WP_000421272.1
Coordinates 112340..112615 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag AVR68_RS00645 Protein ID WP_001387467.1
Coordinates 112056..112340 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR68_RS00615 107519..107959 - 441 Protein_124 zinc-binding dehydrogenase -
AVR68_RS00625 108360..109250 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
AVR68_RS00630 109275..109655 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
AVR68_RS00635 109688..110653 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
AVR68_RS00640 110699..111451 - 753 WP_000562172.1 IclR family transcriptional regulator -
AVR68_RS00645 112056..112340 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
AVR68_RS00650 112340..112615 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AVR68_RS00655 112721..113014 + 294 WP_001105060.1 hypothetical protein -
AVR68_RS29600 113049..113177 - 129 Protein_132 IS481 family transposase -
AVR68_RS00660 113210..114379 - 1170 WP_001072355.1 hypothetical protein -
AVR68_RS00665 114872..115180 + 309 Protein_134 type II toxin-antitoxin system ParD family antitoxin -
AVR68_RS00675 115469..116166 + 698 Protein_135 IS1 family transposase -
AVR68_RS00680 116419..117441 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / tet(A) - 1..126302 126302
- flank IS/Tn - - 115663..116166 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T59427 WP_000421272.1 NZ_CP013833:112340-112615 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T59427 NZ_CP013833:112340-112615 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT59427 WP_001387467.1 NZ_CP013833:112056-112340 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT59427 NZ_CP013833:112056-112340 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References